Phase separation-deficient TDP43 remains functional in splicing.
Schmidt HB, Barreau A, Rohatgi R
Nat Commun. 2019 Oct 25;10(1):4890. doi: 10.1038/s41467-019-12740-2.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107799 | pHBS931 TDP43 RRM-GFP F-S | To test the effect of sequence on TDP43 droplet properties |
| 107800 | pHBS935 TDP43 RRM-GFP M-S | To test the effect of sequence on TDP43 droplet properties |
| 107801 | pHBS934 TDP43 RRM-GFP M-V | To test the effect of sequence on TDP43 droplet properties |
| 107804 | pHBS1037 TDP43 RRM-GFP 6xΦ | To test the effect of sequence on TDP43 droplet properties |
| 107805 | pHBS1038 TDP43 RRM-GFP 4xΦ | To test the effect of sequence on TDP43 droplet properties |
| 107806 | pHBS1039 TDP43 RRM-GFP 2xΦ | To test the effect of sequence on TDP43 droplet properties |
| 107807 | pHBS1256 TDP43 RRM-GFP W385G | To test the effect of sequence on TDP43 droplet properties |
| 107822 | pHBS966 TDP43 RRM-GFP G309F | To test the effect of sequence on TDP43 droplet properties |
| 107824 | pHBS968 TDP43 RRM-GFP G309S | To test the effect of sequence on TDP43 droplet properties |
| 107830 | pHBS1280 TDP43 RRM-GFP G348F | To test the effect of sequence on TDP43 droplet properties |
| 107831 | pHBS1279 TDP43 RRM-GFP G348V | To test the effect of sequence on TDP43 droplet properties |
| 107837 | pHBS1291 TDP43 RRM-GFP G368W | To test the effect of sequence on TDP43 droplet properties |
| 107838 | pHBS1292 TDP43 RRM-GFP G368W+W385G | To test the effect of sequence on TDP43 droplet properties |
| 107856 | pHBS1052 TDP43 RRM-GFP R-K | To test the effect of sequence on TDP43 droplet properties |
| 107857 | pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9 | To knock-out TDP43 |
| 107858 | pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 | To knock-out TDP43 |
| 118791 | pHBS1383 TDP43 RRM-GFP FYW-S | To test the effect of sequence on TDP43 RRM-GFP droplet properties |
| 118792 | pHBS1385 TDP43 RRM-GFP VLIM-S | To test the effect of sequence on TDP43 RRM-GFP droplet properties |
| 118793 | pHBS1384 TDP43 RRM-GFP FYW-L | To test the effect of sequence on TDP43 RRM-GFP droplet properties |
| 118794 | pHBS1386 TDP43 RRM-GFP VLIM-F | To test the effect of sequence on TDP43 RRM-GFP droplet properties |
| 118795 | pHBS1387 TDP43 RRM-GFP KRED-S | To test the effect of sequence on TDP43 RRM-GFP droplet properties |
| 118796 | pHBS1388 TDP43 RRM-GFP K-R | To test the effect of sequence on TDP43 RRM-GFP droplet properties |
| 118797 | pHBS1147 GFP-TDP43 | To test the effect of sequence on nuclear body formation and splicing of full-length TDP43 |
| 118798 | pHBS1084 GFP-TDP43 deltaCTD | To test the effect of sequence on nuclear body formation and splicing of full-length TDP43 |
| 118799 | pHBS1410 GFP-TDP43 F-S | To test the effect of sequence on nuclear body formation and splicing of full-length TDP43 |
| 118800 | pHBS1411 GFP-TDP43 FYW-S | To test the effect of sequence on nuclear body formation and splicing of full-length TDP43 |
| 118801 | pHBS1412 GFP-TDP43 FYW-L | To test the effect of sequence on nuclear body formation and splicing of full-length TDP43 |
| 118802 | pHBS1413 GFP-TDP43 VLIM-F | To test the effect of sequence on nuclear body formation and splicing of full-length TDP43 |
| 118803 | pHBS1389 IBB-GFP-mCherry3E | Fluorescence-based TDP43-dependent splicing reporter |
| 118804 | pHBS1396 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 WT] | To test the effect of sequence on TDP43 splicing activity |
| 118805 | pHBS1393 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaRRM] | To test the effect of sequence on TDP43 splicing activity |
| 118806 | pHBS1394 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaCTD] | To test the effect of sequence on TDP43 splicing activity |
| 118807 | pHBS1392 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaNTD] | To test the effect of sequence on TDP43 splicing activity |
| 118808 | pHBS1401 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S] | To test the effect of sequence on TDP43 splicing activity |
| 118809 | pHBS1404 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-S] | To test the effect of sequence on TDP43 splicing activity |
| 118810 | pHBS1405 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-F] | To test the effect of sequence on TDP43 splicing activity |
| 118811 | pHBS1398 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G368W] | To test the effect of sequence on TDP43 splicing activity |
| 118812 | pHBS1397 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 W385G] | To test the effect of sequence on TDP43 splicing activity |
| 118813 | pHBS1399 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G368W+G385W] | To test the effect of sequence on TDP43 splicing activity |
| 118814 | pHBS1400 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G295F] | To test the effect of sequence on TDP43 splicing activity |
| 121992 | pHBS941 TDP43 RRM-GFP VLIMFYW-S | To test the effect of sequence on TDP43 RRM-GFP droplet properties |
| 133320 | pHBS834 H14-SUMO-TDP43 WT-TEV-mCherry | Bacterial expression of TDP43 with WT CTD |
| 133321 | pHBS1551 H14-SUMO-TDP43 F-S-TEV-mCherry | Bacterial expression of TDP43 with F-S mutant CTD |
| 133322 | pHBS1552 H14-SUMO-TDP43 FYW-S-TEV-mCherry | Bacterial expression of TDP43 with FYW-S mutant CTD |
| 133323 | pHBS1415 GFP-TDP43 VLIM-S | To test the effect of sequence on nuclear body formation and splicing of full-length TDP43 |
| 133324 | pHBS1500 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaNTD] | To test the effect of sequence on TDP43 splicing activity |
| 133325 | pHBS1501 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaRRM] | To test the effect of sequence on TDP43 splicing activity |
| 133326 | pHBS1502 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaCTD] | To test the effect of sequence on TDP43 splicing activity |
| 133327 | pHBS1503 [IBB-GFP-mCherry3E]-[BFP-TDP43 WT] | To test the effect of sequence on TDP43 splicing activity |
| 133328 | pHBS1504 [IBB-GFP-mCherry3E]-[BFP-TDP43 F-S] | To test the effect of sequence on TDP43 splicing activity |
| 133329 | pHBS1505 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-S] | To test the effect of sequence on TDP43 splicing activity |
| 133330 | pHBS1506 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-L] | To test the effect of sequence on TDP43 splicing activity |
| 133331 | pHBS1507 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-S] | To test the effect of sequence on TDP43 splicing activity |
| 133332 | pHBS1508 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-F] | To test the effect of sequence on TDP43 splicing activity |
| 133333 | pHBS1527 [IBB-GFP-mCherry3E]-[BFP-TDP43 W-S] | To test the effect of sequence on TDP43 splicing activity |