Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E, Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 80338 | pTE4495 | mammalian expression MbCpf1 nuclease and Mb crRNA |
| 80339 | pTE4497 | mammalian expression FnCpf1 nuclease and Fn crRNA |
| 80443 | WN10150 | Expresses dead (908A) AsCpf1 |
| 88903 | pTE4565 | Expresses inactive/dead, humanized MbCpf1 nuclease |
| 88904 | pTE4566 | Expresses MbCpf1 crRNA and inactive/dead, humanized MbCpf1 nuclease |
| 88905 | pTE4889 | Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease |
| 107525 | pTE4561 | Expresses Lb crRNA and mCherry in mammalian cells. |
| 107526 | pTE4560 | Expresses As crRNA and mCherry in mammalian cells. |
| 107527 | pTE4938 | Expresses Fn crRNA and mCherry in mammalian cells. |
| 107528 | pTE3179 | Expresses Mb crRNA and mCherry in mammalian cells. |
| 107529 | pTE3320 | Expresses human codon optimized LbCpf1(RVR mutant) in mammalian cells. |
| 107530 | pTE3314 | Expresses human codon optimized FnCpf1(RVR mutant) in mammalian cells. |
| 107531 | pTE3317 | Expresses human codon optimized MbCpf1(RVR mutant) in mammalian cells. |
| 107532 | pTE3316 | Expresses human codon optimized FnCpf1(RR mutant) in mammalian cells. |
| 107533 | pTE3319 | Expresses human codon optimized MbCpf1(RR mutant) in mammalian cells. |
| 107534 | pTE3330 | Expresses Lb crRNA and human codon optimized LbCpf1(RVR mutant) in mammalian cells. |
| 107535 | pTE3327 | Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells. |
| 107536 | pTE3331 | Expresses Fn crRNA and human codon optimized FnCpf1(RVR mutant) in mammalian cells. |
| 107537 | pTE3334 | Expresses Mb crRNA and human codon optimized MbCpf1(RVR mutant) in mammalian cells. |
| 107538 | pTE3329 | Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells. |
| 107539 | pTE3328 | Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells. |
| 107540 | pTE3333 | Expresses Fn crRNA and human codon optimized FnCpf1(RR mutant) in mammalian cells. |
| 107541 | pTE3336 | Expresses Mb crRNA and human codon optimized MbCpf1(RR mutant) in mammalian cells. |
| 107555 | pTE4999 | Expresses human codon optimized inactive FnCpf1(dead1) in mammalian cells. |
| 107557 | pTE4567 | Expresses human codon optimized inactive MbCpf1(dead2) in mammalian cells. |