Paracrine Induction of HIF by Glutamate in Breast Cancer: EglN1 Senses Cysteine.
Briggs KJ, Koivunen P, Cao S, Backus KM, Olenchock BA, Patel H, Zhang Q, Signoretti S, Gerfen GJ, Richardson AL, Witkiewicz AK, Cravatt BF, Clardy J, Kaelin WG Jr
Cell. 2016 Jun 30;166(1):126-39. doi: 10.1016/j.cell.2016.05.042.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118701 | pDONR223-SLC7A11 sh926R | entry clone for SLC7A11 insensitive to hairpin TRCN0000288926 |
| 118702 | pLenti CMV-SLC7A11-sh926R-FLAG-IRES-Hygro | lentiviral vector experessing SLC7A11 insensitive to hairpin TRCN0000288926 |
| 118704 | pLKO.1-TetON-Puro-HIF1a 0819 | Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible) |
| 118705 | pLKO.1-TetON-Puro-HIF1a 3809 | Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible) |
| 118706 | pLenti HRE-Luc pGK Hygro | reporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferase |
| 118707 | pLenti HRE mutant-Luc pGK Hygro | reporter plasmid, HIF-responsive promoter (3xHRE) mutant- fused to firefly luciferase |
| 118708 | pDONR223 NRF2 | entry clone for NRF2 |