A Transcription Factor Addiction in Leukemia Imposed by the MLL Promoter Sequence.
Lu B, Klingbeil O, Tarumoto Y, Somerville TDD, Huang YH, Wei Y, Wai DC, Low JKK, Milazzo JP, Wu XS, Cao Z, Yan X, Demerdash OE, Huang G, Mackay JP, Kinney JB, Shi J, Vakoc CR
Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118636 | Lenti_SaCRISPR_GFP | To clone sgRNA for SaCas9 (SauCas9) |
| 118650 | Lenti_sgRNA_MS2_neo | To clone sgRNA for activation dCas9 |
| 118661 | LentiV_Neo_WT_ZFP64 | Express human wild type ZFP64 gene |
| 118692 | pHAGE_puro | Empty vector to express overexpression cDNA |
| 118693 | pHAGE_puro_ZFP64_N_3XFLAG | Express 3xflag tagged ZFP64 for flag ChIP experiments |
| 118694 | Lenti_Neo_WT_ZFP64_CR | Express sgZFP64e2 resistant cDNA |
| 118695 | pGL421_MLL_promoter | Express MLL/KMT2A promoter for luciferase assay |
| 118696 | pGL421_MLL2_promoter | Express MLL2/KMT2B promoter for luciferase assay |
| 118698 | LentiV_Neo_ZFP64_Delta_TAD | Express ZFP64 without transcription activation domain(TAD) |
| 118699 | Lenti_MS2_P65_HSF1_2A_puro | To express MS2-P65-HSF1 |
| 118700 | Lenti_Neo_ZFP64_N_3XFLAG | Express 3xflag tagged ZFP64 |