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Accurate delineation of cell cycle phase transitions in living cells with PIP-FUCCI.

Grant GD, Kedziora KM, Limas JC, Cook JG, Purvis JE
Cell Cycle. 2018;17(21-22):2496-2516. doi: 10.1080/15384101.2018.1547001. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
118616pLenti-PGK-Neo-PIP-FUCCILentiviral expression of the cell cycle marker PIP-FUCCI
118617pLenti-PGK-Puro-TK-NLS-mTurq2-PCNALentiviral expression of mTurq2 tagged PCNA
118619pLenti-PGK-Neo-PIP-NLS-mVenusLentiviral expression of the cell cycle marker PIP-NLS-mVenus
118621pENTR-PIP-FUCCIGateway cloning vector for the cell cycle indicator PIP-FUCCI
138714pLenti-PGK-Hygro-PIP-FUCCILentiviral expression of the cell cycle marker PIP-FUCCI
138715pLenti-CMV-Blast-PIP-FUCCILentiviral expression of the cell cycle marker PIP-FUCCI

Antibodies from Article