Alternative 3' UTRs direct localization of functionally diverse protein isoforms in neuronal compartments.
Ciolli Mattioli C, Rom A, Franke V, Imami K, Arrey G, Terne M, Woehler A, Akalin A, Ulitsky I, Chekulaeva M
Nucleic Acids Res. 2018 Dec 22. pii: 5258023. doi: 10.1093/nar/gky1270.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
118609 | pMCh1809_pBS_cdc42E6-boxB | cdc42E6 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown |
118612 | pMCh1807_pBS_cdc42E7-boxB | cdc42E7 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown |
118614 | pMCh1750_S2F-IMCg_doxy-CMV_mChe-Cdc42E7 | expression of mChe-tagged cdc42E7 under doxy-inducible CMV promoter |
118615 | pMCh1753_S2F-IMCg_doxy-CMV_mChe-Cdc42E7-E63'UTR | expression of mChe-tagged cdc42E7-E6-3'UTR (swap) under doxy-inducible CMV promoter |
118620 | pMCh2007_pLenti_Syn_mChe-cdc42E7 | expression of mChe-tagged cdc42E7 under pSyn promoter for primary neurons |
118622 | pMCh2008_pLenti_Syn_mChe-cdc42E7-E63'UTR | expression of mChe-tagged cdc42E7-E6-3'UTR under pSyn promoter for primary neurons |