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Alternative 3' UTRs direct localization of functionally diverse protein isoforms in neuronal compartments.

Ciolli Mattioli C, Rom A, Franke V, Imami K, Arrey G, Terne M, Woehler A, Akalin A, Ulitsky I, Chekulaeva M
Nucleic Acids Res. 2018 Dec 22. pii: 5258023. doi: 10.1093/nar/gky1270. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
118609pMCh1809_pBS_cdc42E6-boxBcdc42E6 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown
118612pMCh1807_pBS_cdc42E7-boxBcdc42E7 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown
118614pMCh1750_S2F-IMCg_doxy-CMV_mChe-Cdc42E7expression of mChe-tagged cdc42E7 under doxy-inducible CMV promoter
118615pMCh1753_S2F-IMCg_doxy-CMV_mChe-Cdc42E7-E63'UTRexpression of mChe-tagged cdc42E7-E6-3'UTR (swap) under doxy-inducible CMV promoter
118620pMCh2007_pLenti_Syn_mChe-cdc42E7expression of mChe-tagged cdc42E7 under pSyn promoter for primary neurons
118622pMCh2008_pLenti_Syn_mChe-cdc42E7-E63'UTRexpression of mChe-tagged cdc42E7-E6-3'UTR under pSyn promoter for primary neurons

Antibodies from Article