Efficient isolation of specific genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR.
Fujita T, Fujii H
Biochem Biophys Res Commun. 2013 Sep 13;439(1):132-6. doi: 10.1016/j.bbrc.2013.08.013. Epub 2013 Aug 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 47948 | 3xFLAG-dCas9/pCMV-7.1 | Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest. |
| 61079 | gRNA-hIRF-1 #12 | Expresses a guide RNA (gRNA) to target human IRF-1 promoter |