A Modified Monomeric Red Fluorescent Protein Reporter for Assessing CRISPR Activity.
Hojland Knudsen C, Asgrimsdottir ES, Rahimi K, Gill KP, Frandsen S, Hvolbol Buchholdt S, Chen M, Kjems J, Febbraro F, Denham M
Front Cell Dev Biol. 2018 May 15;6:54. doi: 10.3389/fcell.2018.00054. eCollection 2018.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118413 | pGEmCherry2 | Red fluorescent reporter for assessing CRISPR gRNA activity |
| 118414 | hAAVS1-gs4_EF1a-Cas9-2A-GFP | CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus |
| 118415 | pCAG-SpCas9-2A-GFP-noITR | Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression |
| 118416 | LentiCRISPRv2-sgSORCS2-G2 | CRISPR knockout, expresses Cas9, and sgRNA targeting Human SORCS2 |