Single-fluorophore biosensors for sensitive and multiplexed detection of signalling activities.
Mehta S, Zhang Y, Roth RH, Zhang JF, Mo A, Tenner B, Huganir RL, Zhang J
Nat Cell Biol. 2018 Sep 24. pii: 10.1038/s41556-018-0200-6. doi: 10.1038/s41556-018-0200-6.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118405 | pcDNA3.1(+)-ExRai-AKAR | cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase A activity. |
| 118406 | pcDNA3.1(+)-ExRai-AKAR(T/A) | Negative-control mutant for ExRai-AKAR biosensor. |
| 118407 | pcDNA3.1(+)-ExRai-AKAR-NES | Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity. |
| 118408 | pcDNA3.1(+)-ExRai-AKAR-Kras | Plasma membrane-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity. |
| 118409 | pcDNA3-ExRai-CKAR | cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity. |
| 118410 | pcDNA3-ExRai-CKAR(T/A) | Negative-control mutant for ExRai-CKAR biosensor. |
| 118462 | pcDNA3-ExRai-AktAR | cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity. |
| 118463 | pcDNA3-ExRai-AktAR(T/A) | Negative-control mutant for ExRai-AktAR biosensor. |
| 118464 | pcDNA3.1(+)-sapphireAKAR | cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase A activity. |
| 118465 | pcDNA3.1(+)-sapphireAKAR(T/A) | Negative-control mutant for sapphireAKAR biosensor. |
| 118466 | pcDNA3-Lyn-sapphireAKAR | cp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane. |
| 118467 | pcDNA3.1(+)-sapphireAKAR-NLS | cp-T-sapphire-based single-color PKA biosensor targeted to the nucleus. |
| 118468 | pcDNA3-sapphireCKAR | cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity. |
| 118469 | pcDNA3-sapphireCKAR(T/A) | Negative-control mutant for sapphireCKAR biosensor. |
| 118470 | pcDNA3.1(+)-blueAKAR | cpBFP-based single-color biosensor for monitoring Protein Kinase A activity. |
| 118471 | pcDNA3.1(+)-blueAKAR(T/A) | Negative-control mutant for blueAKAR biosensor. |
| 118472 | pcDNA3-blueCKAR | cpBFP-based single-color biosensor for monitoring Protein Kinase C activity. |
| 118473 | pcDNA3-blueCKAR(T/A) | Negative-control mutant for blueCKAR biosensor. |
| 118474 | pcDNA3-RAB-EKARev | ddRFP-based single-color biosensor for monitoring ERK activity. |
| 118475 | pcDNA3-RAB-EKARev(T/A) | Negative-control mutant for RAB-EKARev biosensor. |
| 118476 | pcDNA3-RAB-EKARev-NLS | ddRFP-based single-color ERK biosensor targeted to the nucleus. |
| 118477 | pcDNA3-Lyn-RAB-EKARev | ddRFP-based single-color ERK biosensor targeted to the plasma membrane. |
| 118478 | pcDNA3-RAB-AKARev | ddRFP-based single-color biosensor for monitoring PKA (Protein Kinase A) activity. |
| 118479 | pcDNA3-RAB-AKARev(T/A) | Negative-control mutant for RAB-AKARev biosensor. |
| 118480 | pcDNA3-RAB-ICUE | ddRFP-based single-color biosensor for monitoring cAMP dynamics. |
| 118482 | pcDNA3.1(+)-PKI-GFP | GFP-tagged Protein Kinase A inhibitor peptide. |