An inducible genome editing system for plants.
Wang X, Ye L, Lyu M, Ursache R, Loytynoja A, Mahonen AP
Nat Plants. 2020 Jun 29. pii: 10.1038/s41477-020-0695-2. doi: 10.1038/s41477-020-0695-2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118385 | p221z-CAS9p-t35s | Entry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system |
| 118386 | p221z-CAS9p-TagRFP-t35s | Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system |
| 118387 | p221z-dCAS9p-t35s | Entry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system |
| 118388 | p221z-AtMIR390a | Entry clone containing AtMIR390a backbone (split by ccdB gene). Used to make artificial microRNA construct. For use in plants and compatible with the MultiSite Gateway system. |
| 118389 | p2R3z-BASI-ccdB-BSAI | Entry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway system |
| 118390 | p2R3z-AtU3b-tRNA-ccdB-gRNA | Entry clone expressing gRNA. Target site can be introduced between tRNA and gRNA backbone. For use in plants and compatible with the MultiSite Gateway system |
| 133747 | pFG7m34GW | Three-fragment Multisite Gateway vector construction for green fluorescent seed selection in Arabidopsis thaliana |
| 133748 | pFRm43GW | Three-fragment Multisite Gateway vector construction for red fluorescent seed selection in Arabidopsis thaliana |