PDSM, a motif for phosphorylation-dependent SUMO modification.
Hietakangas V, Anckar J, Blomster HA, Fujimoto M, Palvimo JJ, Nakai A, Sistonen L
Proc Natl Acad Sci U S A. 2006 Jan 3;103(1):45-50. doi: 10.1073/pnas.0503698102. Epub 2005 Dec 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118352 | hGATA-1 WT | This plasmid expresses human GATA-1 WT. |
| 118353 | hGATA-1 K137R | This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137. |
| 118354 | hMEF2A WT | This plasmid expresses human MEF2A-1 WT. |
| 118355 | hMEF2A K403R | This plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403. |
| 118356 | hHSF4b WT | This plasmid expresses human HSF4b WT. |
| 118357 | hHSF4b K294R | This plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation. |
| 118358 | Gal4-HSF4b WT | This plasmid expresses amino-acids 200-493 of hHSF4b WT fused to the GAL-4 DNA binding domain. |
| 118359 | Gal4-HSF4b K294R | This plasmid expresses amino-acids 200-493 of hHSF4b K294R mutant fused to the GAL-4 DNA binding domain. This construct cannot undergo sumoylation on K294. |