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Dyrk kinases regulate phosphorylation of doublecortin, cytoskeletal organization, and neuronal morphology.

Slepak TI, Salay LD, Lemmon VP, Bixby JL
Cytoskeleton (Hoboken). 2012 Jul;69(7):514-27. doi: 10.1002/cm.21021. Epub 2012 Mar 7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
118270pAAV-DYRK1A-2A-GFPExpresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.
118272pAAV-DYRK1A-2A-mCherryExpresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.
118284pAAV-DYRK2-2A-GFPExpresses mouse DYRK2 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.
118285pAAV-DYRK2-2A-mCherryExpresses mouse DYRK2 tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.
118286pAAV-DYRK3-2A-GFPExpresses mouse DYRK3 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.
118287pAAV-DYRK3-2A-mCherryExpresses mouse DYRK3 tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.
118288pAAV-DYRK4-2A-GFPExpresses mouse DYRK4 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.
118289pAAV-DYRK4-2A-mCherryExpresses mouse DYRK4 tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.
118290pAAV-DCX-2A-mCherryExpresses human DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.
118291pAAV-DCX(S306A)-2A-mCherryExpresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for alanine
118292pAAV-DCX(S306D)-2A-mCherryExpresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for aspartic acid
177166pAAV-DYRK1A(K188R)-2A-mCherryExpresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Lysine at position 188 substituted with Arginine.

Antibodies from Article