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Fluorescent Proteins, Promoters, and Selectable Markers for Applications in the Lyme Disease Spirochete Borrelia burgdorferi

Takacs CN, Kloos ZA, Scott M, Rosa PA, Jacobs-Wagner C
Appl Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 10.1128/AEM.01824-18. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
118225pBSV2G_2Empty E. coli - B. burgdorferi shuttle vector, gentamicin resistant
118226pBSV2_2Empty E. coli - B. burgdorferi shuttle vector; kanamycin resistant
118227pKFSS1_2Empty E. coli - B. burgdorferi shuttle vector; streptomycin resistant
118228pBSV2BEmpty E. coli - B. burgdorferi shuttle vector, blasticidin S resistant
118230pBSV2G_PflaB-mCherryBbGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCherry
118231pBSV2G_PflaB-msfGFPBbGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfGFP
118232pBSV2G_PflaB-msfCFPBbGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfCFP
118233pBSV2G_PflaB-msfYFPBbGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfYFP
118234pBSV2G_PflaB-mEGFPBbGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mEGFP
118235pBSV2G_PflaB-mCeruleanBbGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCerulean
118236pBSV2G_PflaB-mCitrineBbGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCitrine
118237pBSV2G_PflaB-iRFPBbGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven iRFP
118238pBSV2_PresT-mCherryBbKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes resT promoter driven mCherry
118239pBSV2_P0026-mCherryBbKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0026 promoter driven mCherry
118240pBSV2_P0031-mCherryBbKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0031 promoter driven mCherry
118241pBSV2_P0526-mCherryBbKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0526 promoter driven mCherry
118242pBSV2_P0826-mCherryBbKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0826 promoter driven mCherry
118243pBSV2_PflaB-mCherryBbKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCherry
118244pBSV2G_P0826-BB0323-mCherryBbKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoter

Antibodies from Article