Human pancreatic islet three-dimensional chromatin architecture provides insights into the genetics of type 2 diabetes.
Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 10.1038/s41588-019-0457-0.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118152 | pScaffold-H1 donor | PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter |
| 118153 | pSpCas9(BB)-T2A-HygR | SpCas9 expression vector |
| 118155 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR | Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences. |
| 118161 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR BFP-guide1 | CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the BFP CDS |
| 118162 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1 | CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDS |
| 118163 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide2 | CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA2 against the EGFP CDS |
| 118164 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide3 | CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA3 against the EGFP CDS |
| 118165 | lentiSAMv2 BFP-guide1 | CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA1 against the BFP CDS |
| 118166 | lentiSAMv2 EGFP-guide1 | CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA1 against the EGFP CDS |
| 118167 | lentiSAMv2 EGFP-guide2 | CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA2 against the EGFP CDS |
| 118168 | lentiSAMv2 EGFP-guide3 | CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA3 against the EGFP CDS |
| 118169 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide1 | CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 118170 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide2 | CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 118171 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide3 | CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 118172 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide4 | CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 118173 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide1 | CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 118174 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide2 | CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 118175 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide3 | CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 118176 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide4 | CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 118177 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide1 | CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 118178 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide2 | CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 118179 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide3 | CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 118180 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide4 | CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 118181 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR OPTN TSS-guide1 | CRISPR-mediated repression of OPTN. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 118182 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR OPTN TSS-guide2 | CRISPR-mediated repression of OPTN. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 118183 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR OPTN TSS-guide3 | CRISPR-mediated repression of OPTN. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 118184 | lentiSAMv2 TCF7L2 TSS-guide1 | CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 118185 | lentiSAMv2 TCF7L2 TSS-guide2 | CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 118186 | lentiSAMv2 TCF7L2 TSS-guide3 | CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 118187 | lentiSAMv2 TCF7L2 TSS-guide4 | CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 118188 | lentiSAMv2 GLIS3 TSS-guide1 | CRISPR-mediated activation of GLIS3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 118189 | lentiSAMv2 GLIS3 TSS-guide2 | CRISPR-mediated activation of GLIS3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 118190 | lentiSAMv2 GLIS3 TSS-guide3 | CRISPR-mediated activation of GLIS3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 118191 | lentiSAMv2 GLIS3 TSS-guide4 | CRISPR-mediated activation of GLIS3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 118192 | lentiSAMv2 CAMK1D TSS-guide1 | CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 118193 | lentiSAMv2 CAMK1D TSS-guide2 | CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 118194 | lentiSAMv2 CAMK1D TSS-guide3 | CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 118195 | lentiSAMv2 CAMK1D TSS-guide4 | CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 118196 | lentiSAMv2 OPTN TSS-guide1 | CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 118197 | lentiSAMv2 OPTN TSS-guide2 | CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 118198 | lentiSAMv2 OPTN TSS-guide3 | CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 118199 | lentiSAMv2 OPTN TSS-guide4 | CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 118200 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7903146-guide3 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus) |
| 118201 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide2 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus) |
| 118202 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide3 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus) |
| 118203 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide4 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus) |
| 118204 | lentiSAMv2 rs7903146-guide3 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus) |
| 118205 | lentiSAMv2 rs11257655-guide2 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus) |
| 118206 | lentiSAMv2 rs11257655-guide3 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus) |
| 118207 | lentiSAMv2 rs11257655-guide4 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus) |
| 125393 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide1 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus) |
| 125394 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7903146-guide1 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus) |
| 125395 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7903146-guide2 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus) |
| 125396 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7903146-guide4 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus) |
| 125397 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs4237150-guide1 | CRISPR-mediated repression of human islet enhancer containing T1D/T2D-associated variant rs4237150 (GLIS3 GWAS locus) |
| 125398 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs4237150-guide3 | CRISPR-mediated repression of human islet enhancer containing T1D/T2D-associated variant rs4237150 (GLIS3 GWAS locus) |
| 125399 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs4237150-guide4 | CRISPR-mediated repression of human islet enhancer containing T1D/T2D-associated variant rs4237150 (GLIS3 GWAS locus) |
| 125400 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh1-guide1 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus) |
| 125401 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh1-guide2 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus) |
| 125402 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh1-guide3 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus) |
| 125403 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh1-guide4 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus) |
| 125404 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh2-guide1 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7163757 (C2CD4A/B GWAS locus) |
| 125406 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh2-guide3 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7163757 (C2CD4A/B GWAS locus) |
| 125407 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh2-guide4 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7163757 (C2CD4A/B GWAS locus) |
| 125408 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh3-guide1 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7163757 (C2CD4A/B GWAS locus) |
| 125409 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh3-guide2 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus) |
| 125410 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh3-guide3 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus) |
| 125411 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh3-guide4 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus) |
| 125412 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VPS13C-TSS-guide1 | CRISPR-mediated repression of VPS13C. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125414 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VPS13C-TSS-guide3 | CRISPR-mediated repression of VPS13C. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125415 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VPS13C-TSS-guide4 | CRISPR-mediated repression of VPS13C. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125416 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4A-TSS-guide1 | CRISPR-mediated repression of C2CD4A. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125417 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4A-TSS-guide2 | CRISPR-mediated repression of C2CD4A. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125418 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4A-TSS-guide3 | CRISPR-mediated repression of C2CD4A. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125419 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4A-TSS-guide4 | CRISPR-mediated repression of C2CD4A. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125420 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4B-TSS-guide1 | CRISPR-mediated repression of C2CD4B. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125421 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4B-TSS-guide2 | CRISPR-mediated repression of C2CD4B. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125422 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4B-TSS-guide3 | CRISPR-mediated repression of C2CD4B. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125423 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4B-TSS-guide4 | CRISPR-mediated repression of C2CD4B. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125424 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs1401419-guide1 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs1401419 (CRY2 GWAS locus) |
| 125425 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs1401419-guide2 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs1401419 (CRY2 GWAS locus) |
| 125426 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs1401419-guide3 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs1401419 (CRY2 GWAS locus) |
| 125427 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs1401419-guide4 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs1401419 (CRY2 GWAS locus) |
| 125428 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide1 | CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125429 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide2 | CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125430 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide3 | CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125431 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide4 | CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125432 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR PHF21A-TSS-guide1 | CRISPR-mediated repression of PHF21A. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125433 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR PHF21A-TSS-guide2 | CRISPR-mediated repression of PHF21A. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125434 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR PHF21A-TSS-guide3 | CRISPR-mediated repression of PHF21A. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125435 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR PHF21A-TSS-guide4 | CRISPR-mediated repression of PHF21A. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125436 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs12189774-guide1 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs12189774 (VEGFA GWAS locus) |
| 125437 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs12189774-guide2 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs12189774 (VEGFA GWAS locus) |
| 125438 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs12189774-guide3 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs12189774 (VEGFA GWAS locus) |
| 125439 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs12189774-guide4 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs12189774 (VEGFA GWAS locus) |
| 125441 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VEGFA-TSS-guide2 | CRISPR-mediated repression of VEGFA. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125442 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VEGFA-TSS-guide3 | CRISPR-mediated repression of VEGFA. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125443 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VEGFA-TSS-guide4 | CRISPR-mediated repression of VEGFA. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125444 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7732130-guide1 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7732130 (ZBED3 GWAS locus) |
| 125445 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7732130-guide2 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7732130 (ZBED3 GWAS locus) |
| 125446 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7732130-guide3 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7732130 (ZBED3 GWAS locus) |
| 125447 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7732130-guide4 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7732130 (ZBED3 GWAS locus) |
| 125448 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZBED3-TSS-guide1 | CRISPR-mediated repression of ZBED3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125449 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZBED3-TSS-guide2 | CRISPR-mediated repression of ZBED3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125450 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZBED3-TSS-guide3 | CRISPR-mediated repression of ZBED3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125451 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZBED3-TSS-guide4 | CRISPR-mediated repression of ZBED3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125452 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs58692659-guide1 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs58692659 (ZFAND3 GWAS locus) |
| 125453 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs58692659-guide2 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs58692659 (ZFAND3 GWAS locus) |
| 125454 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs58692659-guide3 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs58692659 (ZFAND3 GWAS locus) |
| 125455 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs58692659-guide4 | CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs58692659 (ZFAND3 GWAS locus) |
| 125456 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZFAND3-guide1 | CRISPR-mediated repression of ZFAND3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125457 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZFAND3-guide2 | CRISPR-mediated repression of ZFAND3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125458 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZFAND3-guide3 | CRISPR-mediated repression of ZFAND3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125459 | Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZFAND3-guide4 | CRISPR-mediated repression of ZFAND3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter. |
| 125460 | lentiSAMv2 rs11257655-guide1 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus) |
| 125461 | lentiSAMv2 rs7903146-guide2 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus) |
| 125462 | lentiSAMv2 rs4237150-guide2 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs4237150 (GLIS3 GWAS locus) |
| 125463 | lentiSAMv2 rs4237150-guide3 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs4237150 (GLIS3 GWAS locus) |
| 125464 | lentiSAMv2 rs4237150-guide4 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs4237150 (GLIS3 GWAS locus) |
| 125465 | lentiSAMv2 C2CD4A/B-enh1-guide1 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus) |
| 125466 | lentiSAMv2 C2CD4A/B-enh1-guide2 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus) |
| 125467 | lentiSAMv2 C2CD4A/B-enh1-guide3 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus) |
| 125468 | lentiSAMv2 C2CD4A/B-enh1-guide4 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus) |
| 125469 | lentiSAMv2 C2CD4A/B-enh2-guide1 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7163757 (C2CD4A/B GWAS locus) |
| 125470 | lentiSAMv2 C2CD4A/B-enh2-guide2 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7163757 (C2CD4A/B GWAS locus) |
| 125471 | lentiSAMv2 C2CD4A/B-enh2-guide3 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7163757 (C2CD4A/B GWAS locus) |
| 125472 | lentiSAMv2 C2CD4A/B-enh2-guide4 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7163757 (C2CD4A/B GWAS locus) |
| 125473 | lentiSAMv2 C2CD4A/B-enh3-guide1 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus) |
| 125474 | lentiSAMv2 C2CD4A/B-enh3-guide2 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus) |
| 125475 | lentiSAMv2 C2CD4A/B-enh3-guide3 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus) |
| 125476 | lentiSAMv2 VPS13C-TSS-guide1 | CRISPR-mediated activation of VPS13C. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125477 | lentiSAMv2 VPS13C-TSS-guide2 | CRISPR-mediated activation of VPS13C. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125478 | lentiSAMv2 VPS13C-TSS-guide3 | CRISPR-mediated activation of VPS13C. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125479 | lentiSAMv2 VPS13C-TSS-guide4 | CRISPR-mediated activation of VPS13C. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125480 | lentiSAMv2 C2CD4A-TSS-guide1 | CRISPR-mediated activation of C2CD4A. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125481 | lentiSAMv2 C2CD4A-TSS-guide2 | CRISPR-mediated activation of C2CD4A. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125482 | lentiSAMv2 C2CD4A-TSS-guide3 | CRISPR-mediated activation of C2CD4A. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125483 | lentiSAMv2 C2CD4A-TSS-guide4 | CRISPR-mediated activation of C2CD4A. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125484 | lentiSAMv2 C2CD4B-TSS-guide1 | CRISPR-mediated activation of C2CD4B. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125485 | lentiSAMv2 C2CD4B-TSS-guide4 | CRISPR-mediated activation of C2CD4B. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125486 | lentiSAMv2 rs1401419-guide1 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs1401419 (CRY2 GWAS locus) |
| 125487 | lentiSAMv2 rs1401419-guide2 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs1401419 (CRY2 GWAS locus) |
| 125488 | lentiSAMv2 rs1401419-guide3 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs1401419 (CRY2 GWAS locus) |
| 125489 | lentiSAMv2 rs1401419-guide4 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs1401419 (CRY2 GWAS locus) |
| 125490 | lentiSAMv2 CRY2-TSS-guide2 | CRISPR-mediated activation of CRY2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125491 | lentiSAMv2 CRY2-TSS-guide3 | CRISPR-mediated activation of CRY2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125492 | lentiSAMv2 PHF21A-TSS-guide1 | CRISPR-mediated activation of PHF21A. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125493 | lentiSAMv2 PHF21A-TSS-guide2 | CRISPR-mediated activation of PHF21A. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125494 | lentiSAMv2 PHF21A-TSS-guide3 | CRISPR-mediated activation of PHF21A. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125495 | lentiSAMv2 PHF21A-TSS-guide4 | CRISPR-mediated activation of PHF21A. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125496 | lentiSAMv2 rs12189774-guide1 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs12189774 (VEGFA GWAS locus) |
| 125497 | lentiSAMv2 rs12189774-guide2 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs12189774 (VEGFA GWAS locus) |
| 125498 | lentiSAMv2 rs12189774-guide3 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs12189774 (VEGFA GWAS locus) |
| 125499 | lentiSAMv2 rs12189774-guide4 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs12189774 (VEGFA GWAS locus) |
| 125500 | lentiSAMv2 VEGFA-TSS-guide1 | CRISPR-mediated activation of VEGFA. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125501 | lentiSAMv2 VEGFA-TSS-guide2 | CRISPR-mediated activation of VEGFA. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125502 | lentiSAMv2 VEGFA-TSS-guide4 | CRISPR-mediated activation of VEGFA. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125503 | lentiSAMv2 rs7732130-guide1 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7732130 (ZBED3 GWAS locus) |
| 125504 | lentiSAMv2 rs7732130-guide2 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7732130 (ZBED3 GWAS locus) |
| 125505 | lentiSAMv2 rs7732130-guide3 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7732130 (ZBED3 GWAS locus) |
| 125506 | lentiSAMv2 ZBED3-TSS-guide1 | CRISPR-mediated activation of ZBED3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125507 | lentiSAMv2 ZBED3-TSS-guide2 | CRISPR-mediated activation of ZBED3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125508 | lentiSAMv2 ZBED3-TSS-guide3 | CRISPR-mediated activation of ZBED3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125509 | lentiSAMv2 rs58692659-guide1 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs58692659 (ZFAND3 GWAS locus) |
| 125510 | lentiSAMv2 rs58692659-guide2 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs58692659 (ZFAND3 GWAS locus) |
| 125511 | lentiSAMv2 rs58692659-guide3 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs58692659 (ZFAND3 GWAS locus) |
| 125512 | lentiSAMv2 rs58692659-guide4 | CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs58692659 (ZFAND3 GWAS locus) |
| 125513 | lentiSAMv2 ZFAND3-guide1 | CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125514 | lentiSAMv2 ZFAND3-guide2 | CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125515 | lentiSAMv2 ZFAND3-guide3 | CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |
| 125516 | lentiSAMv2 ZFAND3-guide4 | CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR. |