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Evolution of a split RNA polymerase as a versatile biosensor platform.

Pu J, Zinkus-Boltz J, Dickinson BC
Nat Chem Biol. 2017 Apr;13(4):432-438. doi: 10.1038/nchembio.2299. Epub 2017 Feb 13. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
118085p5-71N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid
118086p2-39ZB-linker-split C-terminal T7 RNAP expression plasmid
118087p5-79N-29-1 split N-terminal T7 RNAP variant-GGSGSGSS-FRB expression plasmid
118088p5-39FKBP-TSGGSG-Split C-terminal T7 RNAP expression plasmid
118089p6-8rapa-T7- mRNA(GFP)

Antibodies from Article