Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying.
Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young DW, Venken KJT
Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118043 | pUPD3 | GoldenBraid2.0 Universal Part Domesticator #3 |
| 118044 | Alpha1 | GoldenBraid2.0 Alpha 1 destination vector |
| 118045 | Alpha2 | GoldenBraid2.0 Alpha 2 destination vector |
| 118046 | Omega 1 | GoldenBraid2.0 Omega 1 destination vector |
| 118047 | Omega 2 | GoldenBraid2.0 Omega 2 destination vector |
| 118048 | phCMV-IE1 | GBPart - Cytomegalovirus enhancer and promoter for constitutive high level expression in mammalian cells |
| 118049 | pMiniP | GBPart - Minimal Promoter for expression in mammalian cells |
| 118050 | p5xNF-kb_RE | GBPart - Operator/DNA Response element - 5 copies of the NF-kb DNA binding motif |
| 118051 | p4xSMAD_RE | GBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motif |
| 118052 | p5xE-Box | GBPart - Operator/DNA Response element - 5 copies of the E-box motif |
| 118053 | p2xp53_RE | GBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motif |
| 118054 | p6xAP-1_RE | GBPart - Operator/DNA Response element - 6 copies of the AP-1 binding motif |
| 118056 | pELuc | GBPart - ELuc Luciferase CDS |
| 118057 | pRedF | GBPart - RedF Luciferase CDS |
| 118058 | pNLuc | GBPart - NLuc Luciferase CDS |
| 118059 | pRenilla | GBPart - Renilla Luciferase CDS |
| 118060 | pGrRenilla | GBPart - GreenRenilla Luciferase CDS |
| 118061 | pbGH | GBPart - Bovine growth hormone terminator |
| 118062 | hCMV-IE1:ELuc | Transcriptional unit for constitutive expression of ELuc in mammalian cells |
| 118063 | hCMV-IE1:FLuc | Transcriptional unit for constitutive expression of FLuc in mammalian cells |
| 118064 | hCMV-IE1:RedF | Transcriptional unit for constitutive expression of RedF in mammalian cells |
| 118065 | hCMV-IE1:NLuc | Transcriptional unit for constitutive expression of NLuc in mammalian cells |
| 118066 | hCMV-IE1:Renilla | Transcriptional unit for constitutive expression of Renilla in mammalian cells |
| 118067 | hCMV-IE1:GrRenilla | Transcriptional unit for constitutive expression of GrRenilla in mammalian cells |
| 118068 | p(A)n-PAUSE | Transcription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene |
| 118069 | MLRV | Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters |
| 135283 | pCGB | GBPart - CGB Luciferase CDS |
| 135284 | pRoLuc | GBPart - RoLuc Luciferase CDS |
| 135286 | pMetLuc | GBPart - MetLuc Luciferase CDS |
| 135287 | pLucia | GBPart - Lucia Luciferase CDS |
| 135288 | pGLuc | GBPart - GLuc Luciferase CDS |
| 135290 | hCMV-IE1:RoLuc:bgH | Transcriptional unit for constitutive expression of RoLuc in mammalian cells |
| 135292 | hCMV-IE1:MetLuc:bgH | Transcriptional unit for constitutive expression of MetLuc in mammalian cells |
| 135293 | hCMV-IE1:Lucia:bgH | Transcriptional unit for constitutive expression of Lucia luciferase in mammalian cells |
| 135294 | hCMV-IE1:GLuc:bgH | Transcriptional unit for constitutive expression of GLuc in mammalian cells |