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Membrane-bound protein scaffolding in diverse hosts using thylakoid protein CURT1A.

Behrendorff JBYH, Sandoval-Ibanez OA, Sharma A, Pribil M
ACS Synth Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00418. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
117989pN_35S/CTP-mCitrineChloroplast-targeted expression of mCitrine controlled by the CaMV 35S promoter
117990pN_35S/CTP-fluoAChloroplast-targeted expression of CURT_fluoA controlled by the CaMV 35S promoter
117991pN_35S/CTP-fluoBChloroplast-targeted expression of CURT_fluoB controlled by the CaMV 35S promoter
117992pN_35S/CTP-fluoEChloroplast-targeted expression of CURT_fluoE controlled by the CaMV 35S promoter
117993pESC/mCitrine/erg3-mCherryCo-expression of mCitrine under the control of the Gal1 promoter and erg3 fused to mCherry (an ER-targeting marker protein) under the control of the Gal10 promoter
117994pN_35S/mCitrineExpression of mCitrine controlled by the CaMV 35S promoter
117995pN_35S/CURT_flouBExpression of CURT_flouB controlled by the CaMV 35S promoter
117996pESC/mCitrineExpression of mCitrine controlled by the Gal1 promoter
117997pESC/CURT_fluoBExpression of CURT_fluoB controlled by the Gal1 promoter
117998pBAD/CURT_fluoAExpression of CURT_fluoA controlled by the ARA promoter
117999pBAD/CURT_fluoBExpression of CURT_fluoB controlled by the ARA promoter
118000pN_35S/mCitrine/P_UBQ10/Derlin1-mOrange2Co-expression of mCitrine under the control of the CaMV 35S promoter and Derlin1 fused to mOrange2 (an ER-targeting marker protein) under the control of the A. thaliana ubiquitin-10 promoter.
118001pESC/CURT_fluoDExpression of CURT_flouD controlled by the Gal1 promoter

Antibodies from Article