Calcium stabilizes the strongest protein fold.
Milles LF, Unterauer EM, Nicolaus T, Gaub HE
Nat Commun. 2018 Nov 12;9(1):4764. doi: 10.1038/s41467-018-07145-6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 117979 | pET28a-SdrG_N2N3-B1-B2-HIS-ybbr | SdrG N2 and N3, B1 and B2 domains with C-terminal ybbr tag for covalent immobilization |
| 117980 | pET28a-SdrG_N2N3-B1-HIS-ybbr | SdrG N2 and N3, B1 domains with C-terminal ybbr tag for covalent immobilization |
| 117981 | pET28a-MGGG-ybbr-HIS-SdrG_B1-DK | SdrG B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB |
| 117982 | pET28a-MGGG-ybbr-HIS-SdrG_B2-DK | SdrG B2 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB |
| 117983 | pET28a-MGGG-ybbr-HIS-SdrD_B1-DK | SdrD B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB |