Superfolder mTurquoise2(ox) optimized for the bacterial periplasm allows high efficiency in vivo FRET of cell division antibiotic targets.
Meiresonne NY, Consoli E, Mertens LMY, Chertkova AO, Goedhart J, den Blaauwen T
Mol Microbiol. 2019 Apr;111(4):1025-1038. doi: 10.1111/mmi.14206. Epub 2019 Feb 28.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 117930 | sfTq2ox-N1 | Superfolder mTurquoise2 ox (pNM113) |
| 117931 | sfTq2-N1 | Superfolder mTurquoise2 (pNM112) |
| 117932 | sfTq2-C1 | Superfolder mTurquoise2 |
| 117959 | sfTq2-PBP5 | Periplasmic expression of superfolder mTurquoise2 (pNM067 pTHV037-DsbAss-LEGPAGL-sfTq2-EFGS-∆1-17-PBP5) |
| 117960 | sfTq2ox-PBP5 | Periplasmic expression of superfolder mTurquoise2ox (pNM077 pTHV037-DsbAss-LEGPAGL-sfTq2ox-EFGS-∆1-17-PBP5) |
| 124217 | mNG-mTq2 | Cytoplasmic mNeongreen mTurquoise2 tandem, ~60 % energy transfer. pNM043 - pSAV057-mNG-mTq2 |
| 124218 | mNG-sfTq2 | Cytoplasmic mNeongreen superfolder mTurquoise2 tandem, ~60 % energy transfer. pNM044 - pSAV057-mNG-sfTq2 |
| 124219 | mNG-sfTq2ox | Cytoplasmic mNeongreen superfolder mTurquoise2 ox tandem, ~60 % energy transfer. pNM045 - pSAV057-mNG-sfTq2ox |
| 124220 | OmpA-mNG-sfTq2ox | Periplasmic OM bound mNeongreen superfolder mTurquoise2 ox tandem, ~40 % energy transfer. pNM092 - pTHV037-Ompa-SA1-177-(SA-1)-LEDPPAEL-mNG-sfTq2ox, |