Semisynthetic sensor proteins enable metabolic assays at the point of care.
Yu Q, Xue L, Hiblot J, Griss R, Fabritz S, Roux C, Binz PA, Haas D, Okun JG, Johnsson K
Science. 2018 Sep 14;361(6407):1122-1126. doi: 10.1126/science.aat7992.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 117908 | pET51b(+)-NADPH-LUCID-1 (C50 5.1 nM) | Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 5.1 nM |
| 117909 | pET51b(+)-NADPH-LUCID-2 (C50 29 nM) | Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nM |
| 117910 | pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM) | Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uM |
| 117911 | pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM) | Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uM |