Golden Gate based metabolic engineering strategy for wild-type strains of Yarrowia lipolytica.
Egermeier M, Sauer M, Marx H
FEMS Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 10.1093/femsle/fnz022.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 117819 | BB1_12_YlpTEF | Promoter pTEF of Y. lipolytica for GoldenMOCS |
| 117820 | BB1_12_YlpGPD | Promoter pGPD of Y. lipolytica for GoldenMOCS |
| 117821 | BB1_34_YlCyc1TT | Transcription terminator Cyc1TT of Y. lipolytica for GoldenMOCS |
| 117822 | BB1_34_YlMig1TT | Transcription terminator Mig1TT of Y. lipolytica for GoldenMOCS |
| 117823 | BB1_23_YlGUT1 | Encodes glycerol kinase (GUT1) of Y. lipolytica for GoldenMOCS |
| 117824 | pMEG_BB2_YL001 | Transcription unit of Y. lipolytica for GoldenMOCS |
| 117825 | pMEG_BB3_YL001 | Overexpession plasmid of glycerol kinase (GUT1) in Y. lipolytica |
| 117826 | pMEG_YLCas9_empty | CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica |
| 117827 | pMEG_BB3_YL18H_AB | GoldenMOCS backbone for 1 TU in Y. lipolytica |
| 117828 | pMEG_BB3_YL18H_AC | GoldenMOCS backbone for 2 TU in Y. lipolytica |
| 117829 | pMEG_BB3_YL68N_AB | GoldenMOCS backbone for 1 TU in Y. lipolytica |
| 117830 | pMEG_BB3_YL68N_AC | GoldenMOCS backbone for 2 TU in Y. lipolytica |
| 117831 | pMEG_BB3_YL68N_AD | GoldenMOCS backbone for 3 TU in Y. lipolytica |
| 117832 | pMEG_BB3_YL68N_AE | GoldenMOCS backbone for 4 TU in Y. lipolytica |