Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells.
Buser DP, Spiess M
J Vis Exp. 2019 Feb 21;(144). doi: 10.3791/59111.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 117750 | pET24a-VHH-tev-2xTS | Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP, tev cleavage site and His6 epitope |
| 117751 | pET24a-VHH-tev-APEX2 | Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitope |
| 117752 | pET24a-VHH-tev-mCherry | Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP, tev cleavage site and His6 epitope |