Conditional control of fluorescent protein degradation by an auxin-dependent nanobody.
Daniel K, Icha J, Horenburg C, Muller D, Norden C, Mansfeld J
Nat Commun. 2018 Aug 17;9(1):3297. doi: 10.1038/s41467-018-05855-5.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 105937 | pCS2 PCNA GFP | in vitro transcription, cell cycle nuclear marker |
| 105978 | p3E LAP2b | 3' element for Gateway cloning, lamina-associated polypeptide 2 |
| 105979 | bactin:GFP-PCNA | transgenesis, ubiquitous expression |
| 105980 | bactin:GFP-LAP2b | transgenesis, ubiquitous expression |
| 117699 | pCAGGs-NLS-TIR1_P2A_NES-TIR1 | Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA |
| 117713 | pCDNA5FRT/TO_HA-mAID-nanobody | Expression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody) |
| 117714 | pIREShygro HA-mVenus-mAID | Expression of HA-tagged mVenus fused to mAID |
| 117716 | pCS2+_NLS-Tir1-HA | mRNA synthesis of NLS-TIR1-HA for injection |
| 117717 | pCS2+_Flag-myc-NES-Tir1 | mRNA synthesis of Flag-myc-NES-TIR1 for injection |
| 117718 | pCS2+_HA-mAID-nanobody (Kless) | mRNA synthesis of lysine-less HA-mAID-nanobdy for injection |
| 117720 | pIRES_IBB-mCherry-IRES-HA-mAID-nanobody | Expression of nuclear transfection marker IBB-mCherry and HA-tagged mAID-nanobody from one mRNA |