Specific and behaviorally consequential astrocyte Gq GPCR signaling attenuation in vivo with ibetaARK.
Nagai J, Bellafard A, Qu Z, Yu X, Ollivier M, Gangwani MR, Diaz-Castro B, Coppola G, Schumacher SM, Golshani P, Gradinaru V, Khakh BS
Neuron. 2021 Jul 21;109(14):2256-2274.e9. doi: 10.1016/j.neuron.2021.05.023. Epub 2021 Jun 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 117688 | CMV BARK R106A p2A mCherry | CMV-iBARK(R106A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing |
| 117689 | CMV BARK p2A mCherry | CMV-iBARK-p2A-mCherry: Mammalian expression vector for Gaq silencing |
| 117690 | CMV BARK D110A p2A mCherry | CMV-iBARK(D110A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing |
| 117691 | GfaABC1D BARK p2A mCherry | GfaABC1D-iBARK-p2A-mCherry: Viral expression vector for astrocyte Gaq silencing |
| 117692 | GfaABC1D BARK D110A p2A mCherry | GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing |
| 117693 | CAG FLEX BARK p2A mCherry | CAG-FLEx-iBARK-p2A-mCherry: Viral expression vector for Cre-dependent Gaq silencing |
| 117694 | CAG FLEX BARK D110A p2A mCherry | CAG-FLEx-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for Cre-dependent Gaq silencing |