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Adapting dCas9-APEX2 for subnuclear proteomic profiling.

Gao XD, Rodriguez TC, Sontheimer EJ
Methods Enzymol. 2019;616:365-383. doi: 10.1016/bs.mie.2018.10.030. Epub 2018 Dec 10. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
117687pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNAU6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

Antibodies from Article