An improved secretion signal enhances the secretion of model proteins from Pichia pastoris.
Barrero JJ, Casler JC, Valero F, Ferrer P, Glick BS
Microb Cell Fact. 2018 Oct 12;17(1):161. doi: 10.1186/s12934-018-1009-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 117658 | pPICZ-pre-pro-alphaf-E2-Crimson | Test intracellular localisation of E2-Crimson and study the secretion efficiency |
| 117660 | pPICZ-pre-pro-alphaf(I)-E2-Crimson | Test intracellular localisation of E2-Crimson and study the secretion efficiency |
| 117661 | pPICZ-pre-Ost1-alphaf(I)-E2-Crimson | Test intracellular localisation of E2-Crimson and study the secretion efficiency |
| 117662 | pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson | Test intracellular localisation of E2-Crimson and study the secretion efficiency |
| 117663 | pPICZ-pOst1-pro-alphaf(MUT2)-E2-Crimson | Test intracellular localisation of E2-Crimson and study the secretion efficiency |
| 117664 | pPICZ-pre-pro-alphaf-Btl2 | Study secretion efficiency of Btl2 |
| 117665 | pPICZ-pre-Ost1-pro-alphaf-Btl2 | Study secretion efficiency of Btl2 |
| 117667 | pPICZ-pre-Ost1-alphaf(I)-Btl2 | Study secretion efficiency of Btl2 |
| 117668 | pKar2-msGFP-HDEL | Localisation of Endoplasmic Reticulum |
| 117669 | Htb2-GFP | Colorize the nuclei |