Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis.
Castel B, Tomlinson L, Locci F, Yang Y, Jones JDG
PLoS One. 2019 Jan 9;14(1):e0204778. doi: 10.1371/journal.pone.0204778. eCollection 2019.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 117499 | pICSL11015 | FAST-Red selectable marker Golden Gate Level 1 Position 1 |
| 117501 | BCJJ339 | RPS5a:Cas9:E9 Golden Gate Level 1 Position 2 |
| 117502 | BCJJ340 | UBI10:Cas9:E9 Golden Gate Level 1 Position 2 |
| 117503 | BCJJ344 | UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse |
| 117504 | BCJJ345 | YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse |
| 117505 | BCJJ358 | RPS5a:Cas9:E9 Golden Gate Level 1 Position 2 reverse |
| 117506 | pICSL12015 | AtUBI10 promoter Golden Gate Level 0 |
| 117507 | LBJJ433 | AtEC1.2 promoter Golden Gate Level 0 |
| 117508 | LBJJ432 | AtEC_enh. promoter Golden Gate Level 0 |
| 117509 | BCJJ223 | AtMGE1 promoter Golden Gate Level 0 |
| 117510 | pICSL12022 | AtAG promoter Golden Gate Level 0 |
| 117511 | pICSL12021 | AtICU2 promoter Golden Gate Level 0 |
| 117512 | BCJJ163 | AtRPS5a promoter Golden Gate Level 0 |
| 117513 | LBJJ491 | AtYAO promoter Golden Gate Level 0 |
| 117514 | pICSL90016 | Cas9_2 Golden Gate Level 0 |
| 117515 | BCJJ125 | Cas9_3 (with intron) Golden Gate Level 0 |
| 117516 | BCJJ180 | Cas9_4 Golden Gate Level 0 |
| 117517 | LBJJ383 | PCR template for sgRNA classic with polyT as terminator |
| 117518 | LBJJ382 | Also known as LBJJ429. PCR template for sgRNA-EF with polyT, 67bp or 192bp terminator |
| 117519 | pICSL60004 | rbcS-E9 terminator Golden Gate Level 0 |