Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses.
Feng S, Varshney A, Coto Villa D, Modavi C, Kohler J, Farah F, Zhou S, Ali N, Muller JD, Van Hoven MK, Huang B
Commun Biol. 2019 Sep 17;2:344. doi: 10.1038/s42003-019-0589-x. eCollection 2019.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 117482 | pSFFV_sfCherry3C(1-10) | Lentiviral vector for constitutive expression of sfCherry3C(1-10) |
| 117483 | pSFFV_sfCherry3V(1-10) | Lentiviral vector for constitutive expression of sfCherry3V(1-10) |
| 117484 | pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10) | Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells |
| 117485 | pSFFV-SpyTag-sfCherry2(11)-TagBFP | Expresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cells |
| 117656 | pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher | Bacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters. |