Modernized Tools for Streamlined Genetic Manipulation and Comparative Study of Wild and Diverse Proteobacterial Lineages.
Wiles TJ, Wall ES, Schlomann BH, Hay EA, Parthasarathy R, Guillemin K
MBio. 2018 Oct 9;9(5). pii: mBio.01877-18. doi: 10.1128/mBio.01877-18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 117386 | pXS-sfGFP | pGEN-mcs with a modular sfGFP expression scaffold |
| 117387 | pXS-dTomato | pGEN-mcs with a modular dTomato expression scaffold |
| 117388 | pXS-mPlum | pGEN-mcs with a modular mPlum expression scaffold |
| 117389 | pTn7xTS | Tn7 tagging vector with temperature-sensitive origin of replication |
| 117390 | pTn7xTS-sfGFP | Tn7 tagging vector pTn7xTS with sfGFP expression scaffold |
| 117391 | pTn7xTS-dTomato | Tn7 tagging vector pTn7xTS with dTomato expression scaffold |
| 117392 | pTn7xTS-mPlum | Tn7 tagging vector pTn7xTS with mPlum expression scaffold |
| 117393 | pTn7xKS | Tn7 tagging vector with a LacI-controlled kill switch counterselection system |
| 117394 | pTn7xKS-sfGFP | Tn7 tagging vector pTn7xKS with sfGFP expression scaffold |
| 117395 | pTn7xKS-dTomato | Tn7 tagging vector pTn7xKS with dTomato expression scaffold |
| 117396 | pTn7xKS-mPlum | Tn7 tagging vector pTn7xKS with mPlum expression scaffold |
| 117397 | pAX1 | allelic exchange vector with GFP merodiploid tracker and temperature-sensitive origin of replication |
| 117398 | pAX2 | allelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switch |