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Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis.

Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp V
Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 10.1016/j.cels.2018.08.011. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
117318PB09-TRE-beta-globin-miR-124-EF1a-GFPOverexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron
117319PB09-TRE-beta-globin-miR-ctrl-EF1a-GFPOverexpression of miR-124-surrounding locus (not miR-124) in eurkaryotic cells, encoded in a human beta-globin intron
117320PB09-TRE-ZNF787_G379AOverexpression of ZNF787
117321pSMPUW-miR-124-GFP-PuroOverexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron
117322pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1/2-5'CRISPR-Cas9 for miR-124-1/2, 5'
117323pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-3-5'CRISPR-Cas9 for miR-124-3, 5'
117324pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1-3'CRISPR-Cas9 for miR-124-1, 3'
117325pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-2/3-3'CRISPR-Cas9 for miR-124-2/3, 3'
117326pmiRGLO-miR-124-sponge_rev(neg. CTRL)Dual luciferase assay negative control for miR-124 binding
117327pmiRGLO-miR-124-sponge (pos. CTRL)Dual luciferase assay positive control for miR-124 binding
117328pmiRGLO-CTDSP1-201Dual luciferase assay for miRNA-targeted UTR
117329pmiRGLO-CD164-201Dual luciferase assay for miRNA-targeted UTR
117330pmiRGLO-CERS2-201Dual luciferase assay for miRNA-targeted UTR
117331pmiRGLO-GLIS2-201Dual luciferase assay for miRNA-targeted UTR
117332pmiRGLO-BAX-201Dual luciferase assay for miRNA-targeted UTR
117333pmiRGLO-TP73-201Dual luciferase assay for miRNA-targeted UTR
117334pmiRGLO-PPM1F-201Dual luciferase assay for miRNA-targeted UTR
117335pmiRGLO-CDIP1-201Dual luciferase assay for miRNA-targeted UTR
117336pmiRGLO-ADGRL1-201Dual luciferase assay for miRNA-targeted UTR
117337pmiRGLO-ARF1-214Dual luciferase assay for miRNA-targeted UTR
117338pmiRGLO-ARHGAP4-203Dual luciferase assay for miRNA-targeted UTR
117339pmiRGLO-ASIC1Dual luciferase assay for miRNA-targeted UTR
117340pmiRGLO-ATF5-201Dual luciferase assay for miRNA-targeted UTR
117341pmiRGLO-CALM1-201Dual luciferase assay for miRNA-targeted UTR
117342pmiRGLO-CAMK2B-209Dual luciferase assay for miRNA-targeted UTR
117343pmiRGLO-CRTAP-201Dual luciferase assay for miRNA-targeted UTR
117344pmiRGLO-FAM102A-203Dual luciferase assay for miRNA-targeted UTR
117345pmiRGLO-FAM104A-202Dual luciferase assay for miRNA-targeted UTR
117346pmiRGLO-FKBP1A-204Dual luciferase assay for miRNA-targeted UTR
117347pmiRGLO-GRM4-202Dual luciferase assay for miRNA-targeted UTR
117348pmiRGLO-KIAA2013-201Dual luciferase assay for miRNA-targeted UTR
117349pmiRGLO-LEMD2-201Dual luciferase assay for miRNA-targeted UTR
117350pmiRGLO-LFNG-201_UTRDual luciferase assay for miRNA-targeted UTR
117351pmiRGLO-LFNG-203Dual luciferase assay for miRNA-targeted UTR
117353pmiRGLO-MTMR10-201Dual luciferase assay for miRNA-targeted UTR
117354pmiRGLO-PDK2-203Dual luciferase assay for miRNA-targeted UTR
117355pmiRGLO-PLEKHG5-212Dual luciferase assay for miRNA-targeted UTR
117356pmiRGLO-POLR2E-213Dual luciferase assay for miRNA-targeted UTR
117357pmiRGLO-PTPN18-201Dual luciferase assay for miRNA-targeted UTR
117358pmiRGLO-RASSF4-201Dual luciferase assay for miRNA-targeted UTR
117359pmiRGLO-RGS3-201Dual luciferase assay for miRNA-targeted UTR
117360pmiRGLO-RPAIN-202Dual luciferase assay for miRNA-targeted UTR
117361pmiRGLO-RPLP1-201Dual luciferase assay for miRNA-targeted UTR
117362pmiRGLO-SEMA5B-202Dual luciferase assay for miRNA-targeted UTR
117363pmiRGLO-SEMA5B-211Dual luciferase assay for miRNA-targeted UTR
117364pmiRGLO-SERINC2-202Dual luciferase assay for miRNA-targeted UTR
117365pmiRGLO-SH3GLB2-203Dual luciferase assay for miRNA-targeted UTR
117366pmiRGLO-SPRED2-201Dual luciferase assay for miRNA-targeted UTR
117367pmiRGLO-SRM-201Dual luciferase assay for miRNA-targeted UTR
117369pmiRGLO-SUMF1-201Dual luciferase assay for miRNA-targeted UTR
117371pmiRGLO-TMEM134-201Dual luciferase assay for miRNA-targeted UTR
117372pmiRGLO-TMEM63C-201Dual luciferase assay for miRNA-targeted UTR
117373pmiRGLO-TNFRSF10BDual luciferase assay for miRNA-targeted UTR
117375pmiRGLO-TSPAN15-201Dual luciferase assay for miRNA-targeted UTR
117376pmiRGLO-UBE2G2-202Dual luciferase assay for miRNA-targeted UTR
117377pmiRGLO-UBE2I-202Dual luciferase assay for miRNA-targeted UTR
117378pmiRGLO-ZNF787-203Dual luciferase assay for miRNA-targeted UTR

Antibodies from Article