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Golden Mutagenesis: An efficient multi-site-saturation mutagenesis approach by Golden Gate cloning with automated primer design.

Pullmann P, Ulpinnis C, Marillonnet S, Gruetzner R, Neumann S, Weissenborn MJ
Sci Rep. 2019 Jul 29;9(1):10932. doi: 10.1038/s41598-019-47376-1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
117225pAGM22082_cRedpET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

Antibodies from Article