High-Throughput Allelic Replacement Screening in Bacillus subtilis.
Diebold-Durand ML, Burmann F, Gruber S
Methods Mol Biol. 2019;2004:49-61. doi: 10.1007/978-1-4939-9520-2_5.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 117116 | pET-Gate2 ccdB | Destination vector |
| 117117 | pET-Gate2 mazEF | Destination vector with mazEF toxin antitoxin system |
| 117119 | pJet1.2 cat cassette | Chloramphenicol resistance cassette |
| 117120 | pJet1.2 specR cassette | Spectinomycin resistance cassette |
| 117121 | pJet1.2 ermB cassette | Erythromycin resistance cassette |
| 117122 | pJet1.2 kanR cassette | Kanamycin resistance cassette |
| 117123 | pJet1.2 sfGFP | C-terminal GFP tag |
| 117124 | pJet1.2 Tev-Halo tag | C-terminal Tev Halo-Tag |
| 117126 | pJet1.2 HA6-Tag | C-terminal HA-tag |
| 117127 | pJet1.2 Pk6-tag | C-terminal Pk6-tag |
| 117128 | pJet1.2 Tev-Avi-tag | C-terminal Tev-Avi tag Tag |
| 117129 | pJet1.2 'rncS | smc gene upstream homology region |
| 117130 | pJet1.2 ftsY | FtsY gene cassette |
| 117131 | pJet1.2 sivC | smc downstream homology region |