Genome-wide CRISPR screen identifies TMEM41B as a gene required for autophagosome formation.
Morita K, Hama Y, Izume T, Tamura N, Ueno T, Yamashita Y, Sakamaki Y, Mimura K, Morishita H, Shihoya W, Nureki O, Mano H, Mizushima N
J Cell Biol. 2018 Aug 9. pii: jcb.201804132. doi: 10.1083/jcb.201804132.
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PubMed
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 117413 | pMXs GFP-LC3-RFP | Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance. |
| 117414 | pMRXIP TMEM41B-GFP | Expresses TMEM41B tagged with GFP in mammalian cells |
| 117415 | pMRXIP TMEM41B-3xFLAG | Expresses TMEM41B tagged with 3xFLAG in mammalian cells |
| 117416 | pMRXIP 3xFLAG-VMP1 | Expresses VMP1 tagged with 3xFLAG in mammalian cells |
| 117417 | pMXsIP GFP-VMP1 | Expresses VMP1 tagged with GFP in mammalian cells |