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BioBits Health: Classroom Activities Exploring Engineering, Biology, and Human Health with Fluorescent Readouts.

Stark JC, Huang A, Hsu KJ, Dubner RS, Forbrook J, Marshalla S, Rodriguez F, Washington M, Rybnicky GA, Nguyen PQ, Hasselbacher B, Jabri R, Kamran R, Koralewski V, Wightkin W, Martinez T, Jewett MC
ACS Synth Biol. 2019 May 7. doi: 10.1021/acssynbio.8b00381. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
117048pJL1-neoRIn vitro expression of neoR (kanamycin resistance gene) from the T7 promoter
117050pJL1-aadAIn vitro expression of aadA (streptomycin resistance gene) from the T7 promoter
117051pJL1-SpCas9In vitro expression of S. pyogenes Cas9 from the T7 promoter
117052pJL1-mRFP1 gRNAIn vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

Antibodies from Article