CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live cell imaging of endogenous loci.
Tasan I, Sustackova G, Zhang L, Kim J, Sivaguru M, HamediRad M, Wang Y, Genova J, Ma J, Belmont AS, Zhao H
Nucleic Acids Res. 2018 Jun 15. pii: 5038283. doi: 10.1093/nar/gky501.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 117049 | lenti F9-TetR-EGFP-IRES-PuroR | lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats |
| 118712 | pSP2-48-merTetO-EFS-BLaR | Contains an optimized 48-mer TetO repeat for imaging of desired loci |
| 118713 | pSP2-96-merTetO-EFS-BLaR | Contains an optimized 96-mer TetO repeat for imaging of desired loci |