Transcriptional recording by CRISPR spacer acquisition from RNA.
Schmidt F, Cherepkova MY, Platt RJ
Nature. 2018 Oct;562(7727):380-385. doi: 10.1038/s41586-018-0569-1. Epub 2018 Oct 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 116952 | pFS_0143-pET30-Fusicatenibacter_saccharivorans | Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with classic acquisition readout |
| 116953 | pFS_0160-pET30-Fusicatenibacter_saccharivorans_ARRAY2 | Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with classic acquisition readout |
| 116954 | pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI | Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout |
| 116955 | pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI | Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout |
| 116956 | pFS_0272_pET30_pCat-tetR-Term-ptetA-Rluc-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI | Expresses FsRT-Cas1-Cas2 under pT7lac and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout |
| 116957 | pFS_0345_pET30-RT-CRISPR_Bacteroides_fragilis_strain_S14_ARRAY_1 | Expresses BfRT-Cas1-Cas2 under pT7lac promoter, encodes BfCRISPR array 1, compatible with SENECA acquisition readout |
| 116958 | pFS_0346_pET30-RT-CRISPR_Bacteroides_fragilis_strain_S14_ARRAY_1_RC | Expresses BfRT-Cas1-Cas2 under pT7lac promoter, encodes BfCRISPR array 1 RC, compatible with SENECA acquisition readout |
| 116959 | pFS_0347_pET30-RT-CRISPR_Campylobacter_fetus_subsp._Fetus_ARRAY_1 | Expresses CfRT-Cas1-Cas2 under pT7lac promoter, encodes CfCRISPR array 1, compatible with SENECA acquisition readout |
| 116960 | pFS_0348_pET30-RT-CRISPR_Campylobacter_fetus_subsp._Fetus_ARRAY_1_RC | Expresses CfRT-Cas1-Cas2 under pT7lac promoter, encodes CfCRISPR array 1 RC, compatible with SENECA acquisition readout |
| 116961 | pFS_0349_pET30-RT-CRISPR_Cellulomonas_bogoriensis_69B4_ARRAY_1 | Expresses CbRT-Cas1-Cas2 under pT7lac promoter, encodes CbCRISPR array 1, compatible with SENECA acquisition readout |
| 116962 | pFS_0350_pET30-RT-CRISPR_Cellulomonas_bogoriensis_69B4_ARRAY_1_RC | Expresses CbRT-Cas1-Cas2 under pT7lac promoter, encodes CbCRISPR array 1 RC, compatible with SENECA acquisition readout |
| 116963 | pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1 | Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readout |
| 116964 | pFS_0352_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1_RC | Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1 RC, compatible with SENECA acquisition readout |
| 116965 | pFS_0353_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2 | Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout |
| 116966 | pFS_0354_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2_RC | Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2 RC, compatible with SENECA acquisition readout |
| 116967 | pFS_0355_pET30-RT-CRISPR_Candidatus_Accumulibacter_sp._SK-02_ARRAY_1 | Expresses CaSK-02RT-Cas1-Cas2 under pT7lac promoter, encodes CaCRISPR array 1, compatible with SENECA acquisition readout |
| 116968 | pFS_0356_pET30-RT-CRISPR_Candidatus_Accumulibacter_sp._SK-02_ARRAY_1_RC | Expresses CaSK-02RT-Cas1-Cas2 under pT7lac promoter, encodes CaCRISPR array 1 RC, compatible with SENECA acquisition readout |
| 116969 | pFS_0357_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_1 | Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 1, compatible with SENECA acquisition readout |
| 116970 | pFS_0358_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_1_RC | Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 1 RC, compatible with SENECA acquisition readout |
| 116971 | pFS_0359_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_2 | Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 2, compatible with SENECA acquisition readout |
| 116972 | pFS_0360_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_2_RC | Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 2 RC, compatible with SENECA acquisition readout |
| 116973 | pFS_0361_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_3 | Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 3, compatible with SENECA acquisition readout |
| 116974 | pFS_0362_pET30-RT-CRISPR_Candidatus_Accumulibacter_sp._BA-91_ARRAY_1 | Expresses CaBA-91RT-Cas1-Cas2 under pT7lac promoter, encodes CaCRISPR array 1, compatible with SENECA acquisition readout |
| 116975 | pFS_0363_pET30-RT-CRISPR_Desulfarculus_baarsii_DSM_2075_ARRAY_1 | Expresses DbRT-Cas1-Cas2 under pT7lac promoter, encodes DbCRISPR array 1, compatible with SENECA acquisition readout |
| 116976 | pFS_0364_pET30-RT-CRISPR_Desulfarculus_baarsii_DSM_2075_ARRAY_1_RC | Expresses DbRT-Cas1-Cas2 under pT7lac promoter, encodes DbCRISPR array 1 RC, compatible with SENECA acquisition readout |
| 116977 | pFS_0365_pET30-RT-CRISPR_Desulfarculus_baarsii_DSM_2075_ARRAY_2 | Expresses DbRT-Cas1-Cas2 under pT7lac promoter, encodes DbCRISPR array 2, compatible with SENECA acquisition readout |
| 116978 | pFS_0366_pET30-RT-CRISPR_Desulfarculus_baarsii_DSM_2075_ARRAY_2_RC | Expresses DbRT-Cas1-Cas2 under pT7lac promoter, encodes DbCRISPR array 2 RC, compatible with SENECA acquisition readout |
| 116979 | pFS_0367_pET30-RT-CRISPR_Woodsholea_maritima_ARRAY_1 | Expresses WmRT-Cas1-Cas2 under pT7lac promoter, encodes WmCRISPR array 1, compatible with SENECA acquisition readout |
| 116980 | pFS_0368_pET30-RT-CRISPR_Woodsholea_maritima_ARRAY_1_RC | Expresses WmRT-Cas1-Cas2 under pT7lac promoter, encodes WmCRISPR array 1 RC, compatible with SENECA acquisition readout |
| 116981 | pFS_0369_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_1 | Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 1, compatible with SENECA acquisition readout |
| 116982 | pFS_0370_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_1_RC | Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 1 RC, compatible with SENECA acquisition readout |
| 116983 | pFS_0371_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_2 | Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 2, compatible with SENECA acquisition readout |
| 116984 | pFS_0372_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_2_RC | Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 2 RC, compatible with SENECA acquisition readout |
| 116985 | pFS_0373_pET30-RT-CRISPR_Teredinibacter_turnerae_T8412_ARRAY_1 | Expresses TtRT-Cas1-Cas2 under pT7lac promoter, encodes TtCRISPR array 1, compatible with SENECA acquisition readout |
| 116986 | pFS_0374_pET30-RT-CRISPR_Teredinibacter_turnerae_T8412_ARRAY_1_RC | Expresses TtRT-Cas1-Cas2 under pT7lac promoter, encodes TtCRISPR array 1 RC, compatible with SENECA acquisition readout |
| 116987 | pFS_0375_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_1 | Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 1, compatible with SENECA acquisition readout |
| 116988 | pFS_0376_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_1_RC | Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 1 RC, compatible with SENECA acquisition readout |
| 116989 | pFS_0377_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_2 | Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 2, compatible with SENECA acquisition readout |
| 116990 | pFS_0378_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_2_RC | Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 2 RC, compatible with SENECA acquisition readout |
| 116991 | pFS_0379_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_3 | Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 3, compatible with SENECA acquisition readout |
| 116992 | pFS_0380_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_3_RC | Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 3 RC, compatible with SENECA acquisition readout |
| 116997 | pFS_0385_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_1 | Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 1, compatible with SENECA acquisition readout |
| 116998 | pFS_0386_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_1_RC | Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 1 RC, compatible with SENECA acquisition readout |
| 116999 | pFS_0387_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_2 | Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 2, compatible with SENECA acquisition readout |
| 117000 | pFS_0388_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_2_RC | Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 2 RC, compatible with SENECA acquisition readout |
| 117001 | pFS_0389_pET30-RT-CRISPR_Eubacterium_saburreum_DSM_3986_ARRAY_1 | Expresses EsRT-Cas1-Cas2 under pT7lac promoter, encodes EsCRISPR array 1, compatible with SENECA acquisition readout |
| 117002 | pFS_0390_pET30-RT-CRISPR_Eubacterium_saburreum_DSM_3986_ARRAY_1_RC | Expresses EsRT-Cas1-Cas2 under pT7lac promoter, encodes EsCRISPR array 1 RC, compatible with SENECA acquisition readout |
| 117003 | pFS_0391_pET30-RT-CRISPR_Eubacterium_saburreum_DSM_3986_ARRAY_2 | Expresses EsRT-Cas1-Cas2 under pT7lac promoter, encodes EsCRISPR array 2, compatible with SENECA acquisition readout |
| 117004 | pFS_0392_pET30-RT-CRISPR_Eubacterium_saburreum_DSM_3986_ARRAY_2_RC | Expresses EsRT-Cas1-Cas2 under pT7lac promoter, encodes EsCRISPR array 2 RC, compatible with SENECA acquisition readout |
| 117005 | pFS_0414_pET30_MCS-BBaJ23100-LuxR-B0015term-pLux rR-Fluc-rrnBterm-pCat-tetR-Term-ptetA-Rluc-rrnBterm_FsRT-Cas1-Cas2_ARRAY2_FaqI | Expresses FsRT-Cas1-Cas2 under pT7lac, Fluc under pLuxR and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout |
| 117006 | pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI | Expresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout |