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Mechanical regulation of glycolysis via cytoskeleton architecture.

Park JS, Burckhardt CJ, Lazcano R, Solis LM, Isogai T, Li L, Chen CS, Gao B, Minna JD, Bachoo R, DeBerardinis RJ, Danuser G
Nature. 2020 Feb 12. pii: 10.1038/s41586-020-1998-1. doi: 10.1038/s41586-020-1998-1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
116940pLVX neo PFKP-GFPExpression of PFKP-GFP fusion protein
116941pLVX puro TRIM21-GFPExpression of TRIM21-GFP fusion protein
116942pLVX puro TRIM21 C54Y - GFPExpression of TRIM21 C54Y -GFP fusion
138288pLVX neo PFKP-GFP K281RMammalian Expression, Lentiviral. Expression of human phosphofructokinase platelet isoform PFKP-GFP fusion protein with K281R mutation
138289pLVX neo PFKL-GFPMammalian Expression, Lentiviral. Expression of human phosphofructokinase, liver isoform PFKL-GFP fusion protein
138290pLVX neo PFKL-GFP, K272RMammalian Expression, Lentiviral. Expression of human phosphofructokinase, liver isoform PFKL-GFP, K272R, fusion protein
138293pLVX neo ACTN1-GFPMammalian Expression, Lentiviral. Expression of human alpha-actinin-1-GFP fusion protein
138294pLVX neo ACTN1-GFP, Y246EMammalian Expression, Lentiviral. Expression of human alpha-actinin-1-GFP, Y246E fusion protein
138295pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21gRNA for CRISPR/Cas9 knockout of human TRIM21

Antibodies from Article