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Cellular Dynamics and Genomic Identity of Centromeres in Cereal Blast Fungus.

Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI
MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 10.1128/mBio.01581-19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
116896pFGL1170RhH1:mCherry (nuclear marker)
116897pFGL1079CenpC:eGFP (centromere marker)
116898pFGL1258TetOFF:GFP:CenpA (centromere marker)
116899pFGL1169RCenpC:mCherry (centromere marker)
116900pFGL1260eGFP:TubA (tubulin marker )
116901pFGL1260RmCherry:TubA (tubulin marker)
116902pFGL1344Alp6:Bar:mCherry (SPB/MTOC marker)
118991pFGL1252A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 68bp MCS (multiple cloning site) which contains 12 unique restriction sites.
118992pFGL1252_TetOFF(Hyg)TetOFF (promoter-less) cassette with Hygromycin selection
118993pFGL1252_TetGFP(Hyg)TetOFF controlled GFP-tagging vector with Hygromycin selection
118994pFGL1269RMarker fusion of Bar:mCherry (without start codon)
118995pFGL1259RmCherry (without start and stop codons) in pGFL1010
118996pFGL1259GFP (without start and stop codons) in pFGL1010

Antibodies from Article