Arrayed BUB recruitment modules in the kinetochore scaffold KNL1 promote accurate chromosome segregation.
Vleugel M, Tromer E, Omerzu M, Groenewold V, Nijenhuis W, Snel B, Kops GJ
J Cell Biol. 2013 Dec 23;203(6):943-55. doi: 10.1083/jcb.201307016.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 115896 | pcDNA5-LAP-KNL1-M3 | Used for expression of siRNA-resistant KNL1-M3 (modified codons 258 and 259) from the FRT site of FlpIn cells upon addition of doxycyclin. |
| 115902 | pLacI-LAP | Backbone for expressing LacI-LAP-fused proteins. |