Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex.
Yurkovetskiy L, Guney MH, Kim K, Goh SL, McCauley S, Dauphin A, Diehl WE, Luban J
Nat Microbiol. 2018 Dec;3(12):1354-1361. doi: 10.1038/s41564-018-0256-x. Epub 2018 Oct 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 115803 | pscALPS gag-gfp Blasti | Express gag-gfp fusion driven by SFFV promoter |
| 115804 | pscALPS gfp Blasti | Express gfp driven by SFFV promoter |
| 115805 | pecALPS gag-gfp Blasti | Express gag-gfp fusion driven by EIF1a promoter |
| 115806 | ptcALPS gag-gfp Blasti | Express gag-gfp fusion driven by TK promoter |
| 115807 | pscALPS gag-gfp/deltavpx | SFFV promoter expresses gag-gfp fusion with no ORF after CypA promoter |
| 115808 | pscALPS gag-gfp/vpx | SFFV promoter expresses gag-gfp fusion with CypA promoter driving expression of SIVMAC251 vpx |
| 115809 | HIV-1 LTR-gfp | HIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vpr |
| 115810 | pscALPS- SIV-MAC251 vpx | Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein |
| 115811 | pscALPS SIV-MND2 vpx | Encodes codon optimized 3xFLAG-SIVMND2 Vpx (AY159322) and puromycin resistance protein |
| 115812 | pscALPS-SIV-RCM vpx | Encodes codon optimized 3xFLAG-SIVRCM Vpx (AF349680) and puromycin resistance protein |
| 115813 | pscALPS-SIV-MNE vpx | Encodes codon optimized 3xFLAG-SIVMNE Vpx (U79412) and puromycin resistance protein |
| 115814 | pscALPS-SIV-DRL vpx | Encodes codon optimized 3xFLAG-SIVDRL Vpx (KM378563) and puromycin resistance protein |
| 115815 | pscALPS-SIV-AGI vpx | Encodes codon optimized 3xFLAG-SIVAGI Vpx (HM803690) and puromycin resistance protein |
| 115816 | pscALPS- HIV2-ROD vpx | Encodes codon optimized 3xFLAG-HIV2ROD Vpx (M15390) and puromycin resistance protein |
| 115817 | pscALPS-SIV-MND1 vpr | Encodes codon optimized 3xFLAG-SIVMND1 Vpr (M27470) and puromycin resistance protein |
| 115818 | pscALPS-SIV-LST vpr | Encodes codon optimized 3xFLAG-SIVLST Vpr (AF188116) and puromycin resistance protein |
| 115819 | pscALPS-SIV-AGM-TAN1 vpr | Encodes codon optimized 3xFLAG-SIVAGM Vpr (TAN1) (U58991) with repaired premature stop codon and puromycin resistance protein |
| 115820 | pscALPS-SIV-TAL vpr | Encodes codon optimized 3xFLAG-SIVTAL Vpr (AM182197) and puromycin resistance protein |
| 115821 | pscALPS-SIV-SYK vpr | Encodes codon optimized 3xFLAG-SIVSYK Vpr (L06042) and puromycin resistance protein |
| 115822 | pscALPS-SIV-SAB-92018 vpr | Encodes codon optimized 3xFLAG-SIVSAB Vpr (HQ378594) and puromycin resistance protein |
| 115823 | pscALPS-SIV-DEB vpr | Encodes codon optimized 3xFLAG-SIVDEB Vpr (FJ919724) and puromycin resistance protein |
| 115824 | pscALPS-SIV-ASC vpr | Encodes codon optimized 3xFLAG-SIVASC Vpr (KJ461715) and puromycin resistance protein |
| 115825 | pscALPS-SIV-AGM-VER9063 vpr | Encodes codon optimized 3xFLAG-SIVAGM Vpr (Ver 9063) (L40990) and Puro resistance marker |
| 115826 | pscALPS-SIV-AGM-VERAGM3 vpr | Encodes codon optimized 3xFLAG-SIVAGM Vpr (Ver AGM3) (M30931) and puromycin resistance protein |
| 115827 | pscALPS-SIV-GRV vpr | Encodes codon optimized 3xFLAG-SIVGRV Vpr (M66437) and puromycin resistance protein |
| 115828 | pscALPS-SIV-AGM-MAL vpr | Encodes codon optimized 3xFLAG-SIVAGM Vpr (MAL_ZMB) (LC114462) and puromycin resistance protein |
| 115829 | pscALPS-SIV-DEN vpr | Encodes codon optimized 3xFLAG SIVDEN Vpr (AJ580407) and puromycin resistance protein |
| 115830 | pscALPS-SIV-GSN-CN71 vpr | Encodes codon optimized 3xFLAG SIVGSN-CN71 Vpr (AF468658) and puromycin resistance protein |
| 115831 | pscALPS-SIV-GSN-CN166 vpr | Encodes codon optimized 3xFLAG SIVGSN-CN166 Vpr (AF468659) and puromycin resistance protein |
| 115832 | pscALPS-SIV-SUN-K08 vpr | Encodes codon optimized 3xFLAG-SIVSUN Vpr (FR751162) and puromycin resistance protein |
| 115833 | pscALPS-SIV-CPZ-TAN3 vpr | Encodes codon optimized 3xFLAG-SIVCPZ-TAN3 Vpr (DQ374658) and puromycin resistance protein |
| 115834 | pscALPS-SIV-CPZ-LB7 vpr | Encodes codon optimized 3xFLAG-SIVCPZ-LB7 Vpr (DQ373064) and puromycin resistance protein |
| 115835 | pscALPS-SIV-GOR -vpr | Encodes codon optimized 3xFLAG-SIVGOR Vpr (FJ424871) and puromycin resistance protein |
| 115836 | pscALPS-HIV-1 Group O-vpr | Encodes codon optimized 3xFLAG-HIV1 Group O Vpr (L20571) and puromycin resistance protein |
| 115837 | pscALPS-HIV-1 Group P-vpr | Encodes codon optimized 3xFLAG-HIV1 Group P Vpr (HQ179987) and puromycin resistance protein |
| 115838 | pscALPS-SIV-RCM-vpr | Encodes codon optimized 3xFLAG-SIVRCM Vpr (HM803689) and puromycin resistance protein |
| 115839 | pscALPS-SIV-WRC-vpr | Encodes codon optimized 3xFLAG-SIVWRC Vpr (AM713177) and puromycin resistance protein |
| 115840 | pscALPS-SIV-COL-BWC01 vpr | Encodes codon optimized 3xFLAG-SIVCOLVpr (KF214240) and puromycin resistance protein |
| 115841 | pscALPS-SIV-MUS2-1246-vpr | Encodes codon optimized 3xFLAG-SIVMUS21246 Vpr (EF070329) and puromycin resistance protein |
| 115842 | pcDNA3.1 FLAG-SIV-MAC251-vpx | Encodes codon optimized FLAG tagged SIVMAC251-Vpx |
| 115843 | pcDNA3.1 FLAG- SIV-MAC251-vpx-Q76A | Encodes codon optimized FLAG tagged SIVMAC251-Vpx-Q76A mutant |
| 115844 | pcDNA3.1 FLAG-SIV-RCM-vpx | Encodes codon optimized FLAG tagged SIVRCM-Vpx |
| 115845 | pcDNA3.1 HA-FAM208A | Encodes codon optimized HA tagged FAM208A |
| 115846 | pAPM-D4- miR30-L1221 | Control shRNA KD vector |
| 115847 | pAPM-D4 miR30-AGO1 ts3 | AGO1 knockdown |
| 115848 | pAPM-D4 miR30-HDAC1 ts2 | HDAC1 knockdown |
| 115849 | pAPM-D4 miR30-HDAC1 ts3 | HDAC1 knockdown |
| 115850 | pAPM-D4 miR30-AGO1 ts1 | AGO1 knockdown |
| 115851 | pAPM-D4 miR30-AGO1 ts2 | AGO1 knockdown |
| 115852 | pAPM-D4 miR30-HDAC1 ts1 | HDAC1 knockdown |
| 115853 | pAPM-D4 miR30-AGO2 ts1 | AGO2 knockdown |
| 115854 | pAPM-D4 miR30-AGO2 ts2 | AGO2 knockdown |
| 115855 | pAPM-D4 miR30-AGO2 ts3 | AGO2 knockdown |
| 115856 | pAPM-D4 miR30-AGO3 ts1 | AGO3 knockdown |
| 115857 | pAPM-D4 miR30-AGO3 ts2 | AGO3 knockdown |
| 115858 | pAPM-D4 miR30-AGO3 ts3 | AGO3 knockdown |
| 115859 | pAPM-D4 miR30-SETDB1 ts1 | SETDB1 knockdown |
| 115860 | pAPM-D4 miR30-SETDB1 ts2 | SETDB1 knockdown |
| 115861 | pAPM-D4 miR30-SETDB1 ts3 | SETDB1 knockdown |
| 115862 | pAPM-D4 miR30-TRIM28 ts1 | TRIM28 knockdown |
| 115864 | pAPM-D4 miR30-TRIM28 ts3 | TRIM28 knockdown |
| 115865 | pAPM-D4 miR30-DNMT3a ts1 | DNMT3a knockdown |
| 115866 | pAPM-D4 miR30-DNMT3a ts2 | DNMT3a knockdown |
| 115867 | pAPM-D4 miR30-DNMT3a ts3 | DNMT3a knockdown |
| 115868 | pAPM-D4 miR30-PIWIL2 ts1 | PIWIL2 knockdown |
| 115869 | pAPM-D4 miR30-PIWIL2 ts3 | PIWIL2 knockdown |
| 115870 | pAPM-D4 miR30-PIWIL2 ts2 | PIWIL2 knockdown |
| 115871 | pAPM-D4 miR30-FAM208A ts1 | FAM208a knockdown |
| 115872 | pAPM-D4 miR30-FAM208A ts2 | FAM208a knockdown |
| 115873 | pAPM-D4 miR30-FAM208A ts3 | FAM208a knockdown |
| 115874 | pAPM-D4 miR30-MPHOSPH8 ts1 | MPHOSPH8 knockdown |
| 115875 | pAPM-D4 miR30-MPHOSPH8 ts2 | MPHOSPH8 knockdown |
| 115876 | pAPM-D4 miR30-MPHOSPH8 ts3 | MPHOSPH8 knockdown |
| 115877 | pAPM-D4 miR30-PPHLN1 ts1 | PPHLN1 knockdown |
| 115878 | pAPM-D4 miR30-PPHLN1 ts2 | PPHLN1 knockdown |
| 115879 | pAPM-D4 miR30-PPHLN1 ts3 | PPHLN1 knockdown |
| 115880 | pAPM-D4 miR30-SUV39h1 ts1 | SUV39h1 knockdown |
| 115881 | pAPM-D4 miR30-SUV39h1 ts2 | SUV39h1 knockdown |
| 115882 | pAPM-D4 miR30-SUV39h1 ts3 | SUV39h1 knockdown |
| 115883 | pAPM-D4 miR30-SUV39h2 ts1 | SUV39h2 knockdown |
| 115884 | pAPM-D4 miR30-SUV39h2 ts2 | SUV39h2 knockdown |
| 115885 | pAPM-D4 miR30-SUV39h2 ts3 | SUV39h2 knockdown |
| 115886 | pAPM-D4 miR30-MORC2 ts1 | MORC2 knockdown |
| 115887 | pAPM-D4 miR30-MORC2 ts2 | MORC2 knockdown |
| 115888 | pAPM-D4 miR30-MORC2 ts3 | MORC2 knockdown |
| 128504 | pscALPS_puro | Encodes puromycin resistance protein |
| 128505 | pAPM D4 BsmBI Myd88 stuffer | pAPM knockdown vector with Myd88 stuffer sequence |