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High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing.

Khoury G, Purcell DFJ
J Vis Exp. 2019 Jan 22;(143). doi: 10.3791/58753. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
115775pLTR.gp140/EGFP.Revdelta38/DsRedHIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.
115776pCMV-Rev(NL4.3)pCMV-RevNL4.3 allows the expression of Rev protein.
115777pcDNA3.1-Tat101(AD8)-FlagpCMV-Tat101AD8-Flag allows the expression of Tat101(AD8) protein with a C-terminal flag-tag.

Antibodies from Article