Autoinhibition of DNA cleavage mediated by RAG1 and RAG2 is overcome by an epigenetic signal in V(D)J recombination.
Grundy GJ, Yang W, Gellert M
Proc Natl Acad Sci U S A. 2010 Dec 28;107(52):22487-92. doi: 10.1073/pnas.1014958107. Epub 2010 Dec 13.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 115646 | coreR1 (384-1008) | expression of truncated Rag1 |
| 115647 | UR1Ct (265-1040) | expression of truncated Rag1 including the RING, E3 ubiquitin ligase (265-1008) and C-terminal region (384-1040) |
| 115648 | coreR2 (1-387) | expression of truncated Rag2 |
| 115649 | R2Ct (1-520) | expression of truncated Rag2 including the C-terminal region |
| 115650 | Rag2 T490A | expression of full length Rag2 with T490A mutation |