A synthetic non-histone substrate to study substrate targeting by the Gcn5 HAT and sirtuin HDACs.
Rossl A, Denoncourt A, Lin MS, Downey M
J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 115565 | GFP-X0 | Control vector expressing GFP alone without any consensus repeats |
| 115566 | GFP-X1 | GFP tethered to a single repeat of the Gcn5 consensus motif |
| 115567 | GFP-X2 | GFP tethered to two repeats of the Gcn5 consensus motif |
| 115568 | GFP-X3 | Synthetic acetylation substrate consisting of GFP fusion with 3 repeats of the Gcn5 consensus motif |
| 115569 | GFP-X2R1 | GFP-X2 where the first consensus motif's lysine is mutated to arginine |
| 115570 | GFP-X2R2 | GFP-X2 where the second consensus motif's lysine is mutated to arginine |
| 115571 | GFP-X2DM | GFP-X2 where both of the consensus motif's lysines are mutated to arginine |
| 115572 | Spt2-GFP | Expresses yeast Spt2-GFP fusion protein |
| 115573 | Spt2-K166R-GFP | Expresses yeast Spt2-GFP fusion protein mutated from lysine to arginine at site 166 |