Molecular Toolkit for Gene Expression Control and Genome Modification in Rhodococcus opacus PD630.
DeLorenzo DM, Rottinghaus AG, Henson WR, Moon TS
ACS Synth Biol. 2018 Feb 16;7(2):727-738. doi: 10.1021/acssynbio.7b00416. Epub 2018 Feb 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 119879 | pDD120 | Constitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance. |
| 119880 | pDD143 | Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance. |
| 119882 | pDD57 | Constitutive expression of GFP+ on pAL5000/pMB1 backbone with kanamycin resistance. |
| 119883 | pDD56 | Arabinose inducible EYFP on pAL5000/pMB1 backbone with kanamycin resistance. |
| 119884 | pDD108 | Integration vector into the ROCI-2 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance marker |
| 119885 | pDD132 | Integration vector into the ROCI-3 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance marker |
| 119893 | pDD112 | pNG2 backbone with an optimized chloramphenicol resitance marker for Rhodococcus opacus PD630 |