Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems.
Papikian A, Liu W, Gallego-Bartolome J, Jacobsen SE
Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 115480 | pEG302 5aa SunTag VP64 nog | CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide) |
| 115481 | pEG302 5aa SunTag VP64 gRNA4 (FWA) | CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA |
| 115482 | pEG302 5aa SunTag VP64 gRNA17 (FWA) | CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA |
| 115483 | 5aa SunTag VP64 EVD | CRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each with two guide RNAs |
| 115484 | 5aa SunTag VP64 AP3 | CRISPR-Cas9 SunTag system to target VP64 to the AP3 locus with two guide RNAs |
| 115485 | 5aa SunTag VP64 CLV3 | CRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two guide RNAs |
| 115486 | pEG302 22aa SunTag NtDRMcd gRNA4 (FWA) | CRISPR-Cas9 SunTag system to target NtDRMcd to the FWA locus with a single guide RNA |
| 115487 | pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA) | CRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAs |
| 115488 | pEG302 22aa SunTag NtDRMcd nog | CRISPR-Cas9 SunTag system to target NtDRMcd to specific loci of interest (nog=no guide) |
| 115489 | pEG302 22aa SunTag NtDRMcd SUP | CRISPR-Cas9 SunTag system to target NtDRMcd to the SUPERMAN locus with two guide RNAs |
| 115490 | pEG302 22aa SunTag NtDRMcd (no NLS) SUP | CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAs |
| 117168 | pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA) | CRISPR Cas9 SunTag system to target NtDRMcd (without an NLS) to the FWA locus with three guide RNAs |
| 119554 | pEG302 22aa SunTag NtDRMcd (noNLS) nog | CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide) |
| 119672 | pEG302 5aa SunTag VP64 g4+g17 (FWA) | CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs |
| 120249 | pEG302 22aa SunTag VP64 gRNA4 (FWA) | CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA |
| 120250 | pEG302 22aa SunTag VP64 gRNA17 (FWA) | CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA |
| 120251 | pEG302 22aa SunTag VP64 nog | CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide) |
| 120252 | pEG302 22aa SunTag VP64 g4+g17 | CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs |