An Accessible Continuous-Culture Turbidostat for Pooled Analysis of Complex Libraries.
McGeachy AM, Meacham ZA, Ingolia NT
ACS Synth Biol. 2019 Apr 19;8(4):844-856. doi: 10.1021/acssynbio.8b00529. Epub 2019 Apr 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 115429 | pPGK1-(AscI)-λN-T2A-SpHIS5 (pNTI347) | Selection for in-frame fusion expression constructs |
| 115431 | pRPL28 CaURA3 (pNTI592) | URA3-marked RPS28 |
| 115432 | pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619) | Vector for expressing RPL28 with mutagenized exon2 |
| 115433 | pRPL28 SpHIS5 (pNTI663) | Expresses wild-type RPL28 from HIS3 yeast plasmid |
| 115434 | pRPL28(Q38P) SpHIS5 (pNTI664) | Expresses Q38P RPL28 from HIS3 yeast plasmid |
| 115435 | pRPL28(H39L) SpHIS5 (pNTI665) | Expresses H39L RPL28 from HIS3 yeast plasmid |
| 115436 | pRPL28(K55M) SpHIS5 (pNTI666) | Expresses K55M RPL28 from HIS3 yeast plasmid |