Maturation-driven transport and AP-1-dependent recycling of a secretory cargo in the Golgi.
Casler JC, Papanikou E, Barrero JJ, Glick BS
J Cell Biol. 2019 May 6;218(5):1582-1601. doi: 10.1083/jcb.201807195. Epub 2019 Mar 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 115420 | YIplac204-pOst1-ESCargo | Expresses a regulatable secretory cargo for yeast at the TRP1 locus |
| 115421 | YIplac128-pOst1-ESCargo | Expresses a regulatable secretory cargo for yeast at the LEU2 locus |
| 115422 | YIplac211-Erg11-iGFPx3 | Express Erg11-iGFPx3 at the endogenous locus via pop-in pop-out mutagenesis |
| 115423 | YIplac211-Sec31-yoHalo | Express Sec31-yoHalo at the endogenous locus via pop-in pop-out mutagenesis |
| 115424 | pSec7-iGFPx6 | Express Sec7-iGFPx6 at the endogenous locus via pop-in pop-out mutagenesis |
| 115425 | pSec7-Halox2 | Express Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesis |
| 115426 | YIplac211-vps10-104 | Generate the vps10-104 allele via pop-in pop-out mutagenesis |
| 115427 | YIplac204-pOst1-ESCargo* | Expresses a regulatable secretory cargo (lacking an ER export signal) for yeast at the TRP1 locus |
| 115428 | YIplac204-pOst1-APVNTA-DsRed-Express2-FKBP(LV)(C22V) | Expresses a non-glycosylated regulatable secretory cargo for yeast at the TRP1 locus |
| 160904 | YIplac211-sGFP-Vrg4 | Expresses GFP-Vrg4 in yeast cells |