CRISPR-Cas12a-Assisted Recombineering in Bacteria.
Yan MY, Yan HQ, Ren GX, Zhao JP, Guo XP, Sun YC
Appl Environ Microbiol. 2017 Aug 17;83(17). pii: AEM.00947-17. doi: 10.1128/AEM.00947-17. Print 2017 Sep 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 158706 | pJV53-Cas12a | CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61 |
| 158707 | pMV261-Cas12a | expresses codon-optimized FnCpf1 |
| 158708 | pCR-Hyg | For cloning and constitutive expression of crRNAs in mycobacterium |
| 158709 | pCR-Zeo | For cloning and constitutive expression of crRNAs in mycobacterium |
| 158711 | pAC-crRNA-Cm | Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA |
| 158712 | pAC-crRNA-Km | Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA |