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Visualizing conformational dynamics of proteins in solution and at the cell membrane.

Gordon SE, Munari M, Zagotta WN
Elife. 2018 Jun 20;7. pii: 37248. doi: 10.7554/eLife.37248. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
126627FLAG-MBP1-K295TAG-W340A.pcDNA3-kExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site.
127399FLAG-MBP1-K295TAG-W340A-CAAX.pcDNA3-kExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site
127400FLAG-MBP1-K295TAG-W340A-S233H-T237H.pcDNA3-kExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site
127401FLAG-MBP1-K295TAG-W340A-S233H-T237H-CAAX.pcDNA3-kExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site
127402FLAG-MBP1-K295TAG-W340A-S233H-T237C.pcDNA3-kExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site
127403FLAG-MBP1-K295TAG-W340A-S233H-T237C-CAAX.pcDNA3-kExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site
127404FLAG-MBP1-E322TAG-W340A.pcDNA3-kExpresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site.
127405FLAG-MBP1-E322TAG-W340A-CAAX.pcDNA3-kExpresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site
127406FLAG-MBP1-E322TAG-K305H-E309H-W340A.pcDNA3-kExpresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site
127407FLAG-MBP1-E322TAG-K305H-E309H-W340A-CAAX.pcDNA3-kExpresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site
127408FLAG-MBP1-E322TAG-E309C-W340A.pcDNA3-kExpresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site
127409FLAG-MBP1-E322TAG-E309C-W340A-CAAX.pcDNA3-kExpresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Antibodies from Article