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Timing of ESCRT-III protein recruitment and membrane scission during HIV-1 assembly.

Johnson DS, Bleck M, Simon SM
Elife. 2018 Jul 4;7. pii: 36221. doi: 10.7554/eLife.36221. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
115328pLNCX2-mEGFP-CHMP2Aexpresses mEGFP-CHMP2A in mammalian cells
115329pLNCX2-mEGFP-CHMP2Bexpresses mEGFP-CHMP2B in mammalian cells
115330pLNCX2-mCherry-CHMP2Aexpresses mCherry-CHMP2A in mammalian cells
115331pLNCX2-mCherry-CHMP2Bexpresses mCherry-CHMP2B in mammalian cells
115332pLNCX2-mCherry-CHMP2A-siRNAresexpresses siRNA-resistant mCherry-CHMP2A in mammalian cells
115333pLNCX2-mCherry-CHMP2B-siRNAresexpresses siRNA-resistant mCherry-CHMP2B in mammalian cells
115334pLNCX2-mCherry-VPS4Aexpresses mCherry-VPS4A in mammalian cells
116890Gag-delta-p6-mEGFPexpresses Gag missing the p6 domain, tagged with mEGFP, in mammalian cells

Antibodies from Article